微波照射对兔附睾管周组织超微结构及碱性磷酸酶细胞化学的影响

EFFECTS OF MICROWAVE IRRADIATION ON ULTRASTRUCTURAL AND ALKALINE PHOSPHATASE OF THE PERITUBULAR TISSUE OF DUCTUS EPIDIDYMIDES IN RABBITS

本文用微波(2450mHz)照射兔阴囊,使阴囊皮肤温度升至41~42℃,维持20min。用电镜观察照射后30min至4个月之间不同时间阶段附睾中段的附睾管周组织超微结构改变,同时还观察照射后12h至30d之间不同时间阶段,对附睾管周组织内碱性磷酸酶细胞化学的影响。照射后1/2~1h,即见平滑肌细胞和毛细血管内皮细胞呈现明显的形态学改变。24h~3d后,小管周组织增厚,其中的固有膜增厚内凸明显。3~4个月后,平滑肌细胞一般已恢复正常形态,但小管周组织的增厚和固有膜的内凸仍然存在。碱性磷酸酶分布于基膜、固有膜、最内层平滑肌细胞胞浆及其表面小泡。照射后12h碱性磷酸酶活动无明显改变,15d后明显减少,30d后接近正常。

The scrota and testes of the rabbits were exposed to 2450 mHz microwave field and the temperature of scrotal skin was raised to 41-42℃for 20 minutes.Ultrastructural observations on the peritubular tissue of ductus epididymides were studied at different intervals from 30 minutes to 4 months after the treatment,and alkaline phosphatase cytochemical changes were observed at intervals from 12 hours to 30 days.Pronounced morphological alterations of the smooth muscle cells and endothelial cells of capillaries were detected within 30 minutes to I hour following exposure.After 24 hours to 3 days,the thickening of the peritubular tissue were noted.The thickening and infolding of the proper lamina were also prominent.The degenerative smooth muscle cells generally returned to normal appearance after 3-4 months,but the thickening of the peritubular tissue and infolding of the proper lamina still appeared.Alkaline phosphatase was localized in the basal lamina,proper lamina,the innermost smooth muscle cells cytoplasm and surface vesicles.There were no obvious alterations of alkaline phosphatase activity after 12 hours.After 15 days,the alkaline phosphatase prominently decreased,while after 30 days the alkaline phosphatase increased nearly to normal.