单胺氧化酶和硫胺素焦磷酸酶在豚鼠交感——肾上腺髓质系统细胞超微结构内的定位

ULTRASTRUCTURAL LOCALIZATION OF MONOAMINE OXIDASE AND THAMINE PYROPHOSPHATASE ACTIVITY IN THE CELLS OF SYMPATHO-ADRENAL MEDULLAR SYSTEM IN THE GUINEA PIG

分别采用Shannon和Novikoff法显示单胺氧化酶(MAO)和硫胺素焦磷酸酶(TPPase)活性在豚鼠腹腔神经节和肾上腺髓质内的分布。在细胞水平发现,MAO活性定位在腹腔神经节小强荧光(SIF)细胞的局部核膜、细胞膜以及少数线粒体膜上,但是很少见到TPPase反应产物。在同一神经节的主神经元中,局部核膜、大量线粒体膜和内质网膜上,都显示出MAO活性反应。此外,TPPase广泛分布在它的高尔基体的靠近成熟面的扁囊上。在肾上腺髓质嗜铬细胞内,MAO和TPPase的定位特点与SIF细胞相似。以上结果提示,在生理状态下,SIF细胞内的儿茶酚胺代谢以及细胞活动方式,可能与主神经元不同,而与肾上腺髓质嗜铬细胞有相似的特点。

Monoamine oxidase and thiamine pyrophosphatase activities were demonstrated ultracytochemically in the celiac ganglion and adrenal medulla of the guinea pig by Shannon’s and Novikoff’s method respectively.Monoamine oxidase activity was found frequently on the nuclear evelope,and ocassionally in mitochondrial outer compartment and cell membrane of the small intensely fluorescent(SIF)cells of the ganglion.Similar Iocalizations were also observed in chromaffin cells of the adrenal medulla.In pricipal neurons of the ganglion,a large amount of products of the monoamine oxidase reaction were found on the endoplasmic reticulium in addition to the nuclear envelope and mitochondrial membranes.Abundant thiaminepyrophosphatase activity was seen at the tran-face of the Golgi complex in the pricipal neurons,in contrast to which,both SIF cells and chromaffin cells exhibited little thiamine pyrophosphatase activity.The results suggested that catecholamine metabolism and the mode of functional activity in the SIF cells were different from those in the pricipal neurons but similar to those in the chromaffin cells of adrenal medulla.