家兔脊髓下橄榄投射的突触特点——HRP顺行标记法电镜研究

THE SYNAPTIC CHARACTERISTICS OF THE SPINO-OLIVARY PROJECTION IN THE RABBIT——AN ELECTRON’ MICROSCOPE STUDY WITH THE ANTEROGRADE HORSERADISH PEROXIDASE METHOD

将HRP注入家兔脊髓腰膨大灰质,在对侧下橄榄背侧副核内显示出大量HRP顺行标记的脊髓纤维终末。并用电子显微镜观察这些标记终末构成的突触。标记终末主要含圆形清亮囊泡,少数含扁形或多形清亮囊泡,清亮囊泡间常夹有散在的大致密芯囊泡。在下橄榄背侧副核内,大部分标记终末形成轴树突触,少数形成轴体突触。有一标记终末同时含圆形及扁形两种清亮囊泡,各与一树突及一胞体构成突触。特别是标记终末还参与构成轴轴突触及突触球。本文还对这些特殊结构的机能意义进行了初步探讨。

Horseradish peroxidase(HRP)was injected into the lumbar enlargement of the spinal cord of the rabbit.A lot of anterograde labeled terminals were observed in the contralateral dorsal accessory olivary nucleus.The synapses contacting these labeled terminals were studied with electron microscope.Most of the labeled terminals contained spherical clear vesicals,only a few of them contained flat or pleomorphic cIear vesicles.The labeled terminals were mainly in contact with dendrites,a few contacting with the perikarya.One of the labeled terminals containsd both round and flat clear vesicoles,presynapticto a dendrite and a perikaryonrespectively.It was notabte that a few of the labeled terminals were included in axon-axonal synapses and glumeruli.The functional meaning of these special structures was discussed tentatively.