大鼠中缝大核神经元向额叶皮质、海马、丘脑、小脑及脊髓的分支投射——荧光素双标记法研究

DIVERGENT AXON COLLATERALS FROM THE NUCLEUS RAPHE MAGNUS TO THE FRONTAL CORTEX,HIPPOCAMPUS,THALAMUS,CEREBELLUM AND SPINAL CORD IN THE RAT——A FLUORESCENT RETROGRADE DOUBLE LABELING STUDY

将荧光素FB-NY、PI-Bb及EB-DAPI-Pr分别注射于31只大鼠的额叶皮质、诲马、丘脑、小脑及颈髓,观察中缝大核中荧光素双标记细胞的分布情况。结果表明大鼠中缝大核神经元可分支投射到丘脑—小脑、丘脑—海马、额叶—丘脑、额叶—小脑、海马—小脑、海马—颈髓、小脑—颈髓、丘脑—颈髓以及两侧的海马和丘脑等许多部位。并有一定的局部定位关系。

Different fluorescent tracers,Fast Blue(FB),Nuclear Yellow(NY),Propidium iodide(PI),Bisbenzimide(Bb),Evans Blue(EB),DAPI-Primuline(Pr),were injected into the frontal cortex,hippocampus,thalamus,cerebellum and cervical cord respectively in 31 rats to observe the fluorescent retrograde double labeling cells in the nucleus raphe magnus.The results showed that the axon collaterals of the nucleus raphe magnus projected to the:thalamus-cerebellum,thalamus-hippocampus,frontal cortex-thalamus,frontal cortex-cerebellum,hippocampus-cerebellum,hippocampus-cervical cord,cerebellum-cervical cord,thalamus-cervical cord and bilateral hippocampus and thalamus.The topographical organizations of divergent projection neurons of the nucleus raphe magnus were also found.