两种人体肿瘤细胞系(ECa-109、BEL-7402)和正常中国地鼠卵巢细胞系(CHO)体外浸润能力的研究

A STUDY OF INVADING CAPACITY OF TWO HUMAN TUMOR CELL LINES (ECA-109,BEL-7402) AND NORMAL CHINESE HAMSTER OVARY CELL LINE (CHO)

本文采用双层琼脂体外器官和实验细胞共培养的方法,将人体食管癌细胞系(ECa-109),人体肝癌细胞系(BEL-7402)和正常中国地鼠卵巢细胞系(CHO)对鸡胚中肾和心肌的浸润能力,进行了研究。结果表明:ECa-109细胞和BEL-7402细胞在体外都有浸润能力,ECa-109的浸润能力比BEL-7402强;正常CHO细胞无浸润能力,只能轻微附着靶组织;鸡胚中肾和心肌接受恶性细胞ECa-109和BEL-7402浸润的能力相同。

The method of combining culture of embryonic chicken mesonephrons or heart fragments with some cells on double layer agar-agar medium is used in this experiment.The authors have investigated with this method invading capacity of malignant esophageal cancer cell line(ECa-109),malignant hepatoma cell line(BEL-7402)and normal diploid Chinese hamster ovary cell line(CHO)to embryonic chicken mesonephrons and heart fragments.The authors have found that both ECa-109 cell line and BEL-7402 cell line have invasive capacity in vitro.The invasive capacity of ECa-109 cells is higher than that of BEL-7402 cells.The normal diploid CHO cells can not invade target organs and show only slight adhesion to them.The capacity for ECa-109 cells or BEL-7402 cells to invade both the embryonic chicken mesonephrons and heart fragment is similar.