前列腺良性增生的5′-核苷酸酶和硫胺素焦磷酸酶定位及其活性的观察

LIGHT AND ELECTRON MICROSCOPY OBSERVATION ON ACTIVITIES AND LOCALIZATION OF 5’-NUCLEOTIDASE AND THIAMINE PYROPHOSPHATASE IN THE HUMAN PROSTATE HYPERPLASIA

用光镜和电镜研究了人类前列腺增生的上皮细胞内5′-核苷酸酶(5′-Nase)和硫胺素焦磷酸酶(TPPase)的活性。5′-Nase活性定位分布在上皮细胞的溶酶体、高尔基大泡、小泡、分泌泡以及游离面的质膜,腺腔内分泌物质也呈阳性反应。经克念菌素治疗后,酶活性无明显改变;然而用乙烯雌酚治疗后,酶活性稍弱;睾丸切除后的则完全呈阴性反应。TPPase活性定位在高尔基扁平囊泡的凹面,除睾丸切除后的酶反应完全阴性外,其他各种治疗,对酶活性没有明显改变。

The epithelial cells of human prostatic hyperplasia had been studied cytochemically for 5’-nucleotidase and thiamine pyrophosphatase activity with light and electron microscopy.The 5’-nucleotidase(5’-Nase)activities were present in the epithelial cells at lysosomes,Golgi vacuoles,vesicles,secretory vacuoles and free surface of plasmic membrane,the secretion material within the acinar lumina of the prostate gland also showed 5’-nucleotidase reaction product.The activity of 5’-nucleotidase has not markedly changed by the actinomyces globispores treatment,but slightly decreased after estrogen treatment.It was no enzyme reaction after treated by testectomy.Activities of thiamine pyrophosphatase(TPPase)were restricted to the Golgi area,and there were heavy deposits of reaction products in concave face of Golgi saccule.The activities of TPPase were not markedly changed by estrogen and actinomyces globispores treatments but none of enzyme activities could be seen after testectomy.