金鱼头部瘤样赘生物组织中SDH定位——电镜细胞化学研究

ELECTRON MICROSCOPIC LOCALIZATION OF SUCCINIC DEHYDROGENASE ACTIVITY OF THE TUMOR-LIKE GROWTH TISSUE IN GOLDFISH CARASSIUS AURTUS

本文采用改良的Kerpel-Fronius和Hajós、Wenzel等的电镜细胞化学方法,研究了金鱼头部瘤样赘生物发生变化的组织中的SDH活性分布情况。在10个月龄的金鱼头部瘤样赘生物组织的细胞中,空泡边缘、线粒体膜、内嵴以及核膜上,均呈现出强的阳性反应。质膜上和核内局部,也呈阳性反应。在20个月龄的同类细胞中,空泡边缘、线粒体膜、内嵴及基质中,都呈现出强的阳性反应,核内的反应也比10个月龄的强。在30个月龄的同类细胞中,空泡边缘和核中的阳性反应已基本消失,只有线粒体中和线粒体膜上以及质膜上,仍呈现出阳性反应,但比以前的反应都弱。

In the present study,electron microscopic and cytochemical localization of succinic dehydrogenase(SDH)of the tumor-like growth tissue in the Tiger-head goldfish(Carassius aurtus)were attempted.The SDH was demonstrated according to the modified method described by Kerpel-Fronius and Hajos(1968).The main results are as follows:When the goldfish were twenty-months old,there was not only a highly active positive reaction of SDH on the periphery of jejune vesicle,but also on the mitochondria,cristae and cytoplasm membrane in the cells of the hyperplasitic tissue.At the same time,there were much crystal formation because cell disintegration.When the Tiger-head goldfish were ten and thirty-months old,the active reaction of SDH was not so strong as in the corresponding positions of cytodeme as When it was twenty-months old.The crystal formation were lesser than the others.But the control groups displayed the negative reaction of SDH in the corresponding positions.