人胎儿心肌组织原代培养搏动及其生长特点的观察

THE OBSERVATION ON THE BEATING AND GROWTH CHARACTERISTICS OF THE PRIMARY MYOCARDIAL TISSUE CULTURE OF HUMAN FETUS

本文报道分别取自妊娠10周及16周的胎儿心肌组织块,在一般啮齿动物心肌细胞培养条件下进行培养,出现了自发搏动。搏动频率分别为21~27次/分和200~290次/分。前者连续搏动30天后,人为终止培养。后者中有一细胞团块,连续搏动长达75天,自行停止。由于组织块取材厚薄及形态不同,培养后团块生长方式亦有差别。较厚的组织块生长较慢,以后纤维母细胞及心肌细胞可呈放射状生长,亦可呈索条状向外伸延;边缘较薄的组织块,心肌细胞在早期从边缘呈球形突出,然后脱落生长。较薄层组织块由于周围纤维母细胞很快长满后,心肌组织则不断加厚生长。本文认为心肌组织块培养的优点是操作简便,搏动明显并相对稳定,持续时间较长。但是提高心肌纯度和做心肌细胞计数、定量试验,尚有一定困难。

Myocardial tissue masses were isolated from two human fetus of 10 and 16 week’s gestation respectively and they were cultured under the conditions for cultivating the myocardial cells of the rodents.Spontaneous beating occured.In one case the frequency of beating was 21~27 beats/min,and the beating lasted for 30 days until the culture was terminated artifitially.In another case a cell mass continued to beat as long as 75 days with a frequency of 200~290 beats/min,and then stopped spontaneously.The differences in thickness and shapes of the tissue masses would influence the modes of growth after culturing.Thicker tissue mass grew more slowly and the fibroblasts and myocardial cells grew and spreaded out radially,or in band form.In tissue mass with a thin border the marginal myocardial cells bulged out in globular shape in the early stage and then separated from the cell mass and continued to grow.In thin layered tissue mass,because of the rapid overgrowth of the surrounding fibroblasts,the myocardial tissue would grow and overlap them and increased steadily in thickness.This procedure is simple and relatively stable,and possible to retain the long-lasting beating of the myocardial tissus,but it is difficult to obtain a pure myocardial cell culture for cell counts and other quantitative studies.