豚鼠肝脏微血管铸型扫描电镜观察

OBSERVATION OF THE HEPATIC MICROVASCULAR CASTS OF GUINEA PIGE UNDER THE SCANNING ELECTRON MICROSCOPE

经豚鼠的主动脉灌注低粘度的甲基丙烯酸甲酯,制成肝8庄微血管铸型标本,在双目显微镜下进行解剖,然后镀膜,用扫描电子显微镜观察。在肝脏铸型标本表面,清楚地显示出由肝窦状隙所组成的肝小叶轮廓,在相邻的肝小叶之间,窦状隙的周围部有广泛吻合,放大观察窦状隙的表面,可见有不甚规则而近似圆形的压痕。在肝脏血管铸型的断面标本,可见呈放射状排列的肝窦状隙,它们彼此吻合,并向小叶中心汇集成中央静脉。小叶间Glisson鞘内显示以下结构:小叶间静脉及其与肝窦状隙的吻合支;肝动脉与小叶间静脉伴行,并发出其终末支连于胆管周围丛;胆管周围丛显示为单层毛细血管网,该丛一方面自肝动脉接受其输入血管,另一方面也从该丛发出输出血管,由小叶周围部汇入肝窦状隙,构成胆管周围门脉系统。

Guinea pigs were injected with low viscosity methyl methacrylate through theaorta,and vascular replicas of the liver were prepared.The replicas were dissected under a binocular microscope with insect needle and small forceps;and then they were coated with gold,and observed under scanning electron microscope.The outlines of hepatic lobules consisting of the hepatic sinusoids were clearly shown on the liver surface.There are numerous anastomoses at the peripheral part of sinusoids between the adjacent hepatic lobules.On the surface of casts of sinusoid many spherical impressions were clearly observed.The radiate arrangement of hepatic sinusoids was shown in section of the vascular cast.They anastomose each other and converge into the central vein at the centre of hepatic lobules.In the Glisson’s sheath,following structures were observed;1.Interlobular vein and its branches anastomose with hepatic sinusoids.2.Hepatic artery runs in.company with interlobular vein and sends out terminal branches to the peribiliary plexus.3.Peribiliary plexus consisting of a single network of capillaries receives the afferent vessels from the hepatic artery and its efferent vessels pour into the hepatic sinusoids.