Studies on hematoporphyrin-photosensitized effects on human cancer cells in vitro——TEM and SEM observation

The ECa109 esophageal cancer epithelial cell line cells was treated with hematoporphyrin one hour and followed by irrediation with 20W black light at a distance of two cm for ten minutes.The cancer cells were examined by TEM and SEM after treatment for one,three and five days.By exposure to hematoporphyrin derivative(HpD)and photoirradiation,the proportion of dark-cells with high electron density were decreased and the proportion of empty cells with low electron density was increased.The latter showed distruction of cancer cells.