大白鼠肝癌细胞株FSK7901、FSK7902的超微结构

ULTRASTRUCTURES OF THE HEPATOMA CELL LINES FSK 7901,FSK 7902 OF RAT

本文用大白鼠肝癌细胞株FSK7901、FSK7902体外培养2年多的材料,进行超微结构的研究。细胞的大小不等,散在分布或上皮样排列呈细胞单层。单个散在细胞的外周,有伪足样突起或微绒毛。在细胞单层内,细胞毗邻不紧密处,相邻细胞有短而少的微绒毛;在细胞毗邻紧密处,相邻细胞膜呈波浪型、平直型或杵指镶嵌型。观察到粘合带和粘合斑。细胞核畸形,核仁畸形,体积大、数量多,核仁丝的排列紊乱。细胞质内的RER与线粒体较少,游离核糖体丰富。线粒体与线粒体嵴畸形,体积较小。高尔基氏体多,成群分布于细胞核旁。没有观察到典型RER环层小体和SER环层小体。环孔板易见。观察到PRER结构。核旁嗜酸性区域的超微结构是成群的高尔基氏体或高尔基氏体与线粒体形成。

This paper presents the results of an ultrustructural study of the hepatoma cell lines FSK 7901,FSK 7902 of long term cultures about 2 years more.The size of cell varies.Distribution of the cell is single or in epitheloid monolayer.Pseudopodial processes or microvilli cover the perimeter of certain hepatoma cells.In the monolayer,the microvilli exist where the neighboring plasmalemmae are in loose contact.When their contact is close,the neighboring plasmalemmae may be straight,wavelike or interdigitated.The nucleus and enlarged nucleoli are irregu-lar in shape.The deformity of nucleolonema is remarkable.Rough endoplasmic reticulum(RER)and mitochondria are much less in number in the cytoplasm,but the free ribosomes is very abundant.The shape of mitochondria and their cristae are irregular.The Golgi complex is often or less arranged around 10 in a group,situated near the nucleus.Typical coucentric body from RER or SER has not been observed.Annulate lamellae are easily found.A peculiar structure,primordial rough-surfaced endoplasmic reticulum(PRER)is seen.Juxta-nuclear acidophilic area consists of a group of Goigi complex or Golgi complex and mitochondria.