人类精子与金黄地鼠卵体外受精技术的某些改良

SOME MODIFICATIONS OF IN VITRO FERTILIZATION TECHNIQUE FOR HUMAN SPERMATOZOAL PENETRATION INTO HAMSTER OOCYTES

人类精子与金黄地鼠卵的体外受精技术是近年来建立的一种测定人类精子受精能力(fertilizing capacity)的新方法[1,2],其准确性远较常规精液分析为高[3-5]。目前,国外已将此法用作检测男性不育的一种常规技术[6]。更有意义的是,这种技术也是探索人体早期发生、研究药物等外界因素对受精的影响、以至试管婴儿等重大研究课题的一个重要手段。我们根据美国夏威夷大学解剖系所用的方法加以改良,使之更加简化,便于掌握,也能得到满意的结果。现将我们的操作步骤报道如下。

We describe a modified technique for the in vitro study of human spermatozoal penetration into hamster oocytes which is easier to master and more simple to perform and needs no complicated facilities.It comprises the following aspects:1 The hamster oviduct is stained with 1%neutral red agar slics,so that the opening of the oviduct is easy to find and easy to be inserted with needle for flushing eggs.2.A special small tube,3 cm long with a diameter of 0.5 cm is used for fertilization instead of plastic dish with paraffin oil covering the media.3.Ordinary incubator is used rather than CO_(2)incubator.With these procedures,the sperm penetration rates we got were 75%,25%,40%,80%and 46%respectively in 5 experiments.It could meet the needs to evaluate the fertilizing capacity of human spermatozoa.