针刺镇痛对肾上腺皮质束状带作用的电镜观察

EFFECT OF ACUPUNCTURE ANALGESIA ON ULTRASTRACTURE OF ZONA FASCICULATA OF ADRENAL CORTEX

大鼠肾上腺皮质束状带富含类脂、SER及线粒体,三者是合成皮质激素的主要成分,彼此排列紧密,有利于中间产物的转化与合成。针刺镇痛后,电镜观察见肾上腺皮质内血窦扩张、胞体及核均胀大,特别在束状内带及网状带变化显著,细胞间隙加宽形成小管,并与血窦下间隙相通。细胞邻接面,其中间连接及缝隙连接消失。细胞内线粒体胀大,嵴由管状多变成泡状,巨大线粒体较多,平滑膜泡扩张,腔内含有絮状物,聚核蛋白体增多,类脂滴扩大,密度变浅,高尔基体发达,溶酶体增多。按以上结构分析,针刺镇痛可引起皮质细胞机能活动增强,激素合成及排出增多,亦促进机体的整合作用。

Ten adult male rats of 250~300 g were used.These animals were divided into the control and experimental groups of 6 and 4 rats respectively.The changes of the ultrastructure of adrenocortical cells under acupuncture analgesia were observed with electron microscopy.1.The volumes of cells,nuclei and nucleoli in zona fasciculata were increased.The mitochondria was hypertrophic;its cristae transformed from the tubules into the vesicles in the external zone fasciculate.The giant mitochondria which contained paracrystallin and lipid osmiophilic substance was observed.2.The surface volume of SER membrane was increased.The smooth membrane vesicles and tubules with filaments and granules were dilated.The SER membrane vesicles were arranged around the mitochondria or lipid droplets.3.The lipid droplets with low density were dilated and accumulated in the middle part of cytoplasm.These findings might be correlated with enhancement of synthesis and utilization of cholesterol ester.4.The free ribosomes and polysomes which scattered in cytoplasm were increased.The Golgi apparatus were well developed.The lysosomes of variable sizes and densities(primary or secondary lysosomes)were abundent.5.The number and diameter of the microvilli were increased.The spaces between the adjacent cells of zona fasciculata were dilated and the intermedian junctions and gap junctions had disappeared.The subsinus space was dilated and contained numerous longer microvilli.The changes discribed above suggested that the acupuncture analgesia may enhance activity of the adrenocortical cells,both synthesis and discharge of corticoid hormone.