癌细胞局部接种后引流淋巴结免疫形态学变化的观察

THE OBSERVATION OF IMMUNOMORPHOLOGICAL CHANGES IN THE DRAINING LYMPH NODES OF MICE AFTER IMPLANTATION OF TUMOR CELLS INTO FOOTPAD

本试验对小鼠脚掌皮下接种癌细胞后引起的,以淋巴结为主的淋巴网状组织的反应,进行了组织学及超微结构的检查。癌细胞接种后,无论局部还是非局部,淋巴结反应均很显著。早期以副皮质区体积扩大、免疫母细胞数目增加为主要特点;局部和非局部淋巴结免疫母细胞数目分别在接种后第4天和第7天达到高峰,并以局部增加最为明显。晚期生发中心扩大融合,髓索浆细胞数目增加。中期为早、晚期变化的过渡和移行阶段。提示淋巴结针对肿瘤的免疫反应早期可能是以细胞免疫为主,晚期可能以体液免疫为主,中期细胞免疫继续存在,同时体液免疫开始增强。局部淋巴结在参与肿瘤免疫方面反应早,而且比较强烈,非局部淋巴结在体液免疫方面可能发挥更大作用。透射电镜观察发现,淋巴结内窦组织细胞可以直接吞噬癌细胞,该过程可能需要淋巴细胞辅助。实验结果提示副皮质区免疫母细胞数目增多可作为抗肿瘤细胞免疫的指标,对于带瘤宿主预后判断有重要意义。

The response of murine lymphoreticular tissue with Special reference to regional lymph node after implantation of Ehrlich ascites carcinoma cells was investigated histologically and ultrastructurally.The tumor induced a vigorous histological response in both regional and nonregional lymph nodes that was proliferation of immunoblasts with enlargement of paracortical area in early stage and enlargement,fusion of germinal centers with an increased number of plasma cells in the medullary cords during the late stage of tumor growth.The results suggested that the response of lymph nodes might be mainly related to cell-mediated immune mechanism in the early stage and humoral immunity during the late stage of tumor growth.Regional lymph node gave reaction earlier and more vigorously in participating cell-mediated immunity,nonregional lymph node might be more significantly involved in humoral immunity.It was observed ultrastructurally that sinus histiocyte engulfed tumor cell in which the cooperation with lymphocyte might be necessary.The experimental results indicated that the increased number of immunoblast in paracortex may be considered as an indicator for antitumor cellmediated immunity with an important prognostic significance for host bearing tumor.