豚鼠腹腔神经节小强荧光(SIF)细胞的超微结构及细胞化学研究

AN ULTRASTRUCTURAL STUDY OF SMALL INTENSELY FLUORESCENT(SIF)CELLS IN CELIAC GANGLION OF GUINEA PIG,WITH SPECIAL REFERENCE TO THE CYTOCHEMICAL LOCALIZATION OF ACID PHOSPHATASE

将九只成年豚鼠的腹腔神经节在2%戊二醛和2.5%解聚的多聚甲醛混合液内固定后,切成50~100μm厚片。在荧光显微镜下拣出含有SIF细胞的厚片进行酸性磷酸酶孵育以及电子显微镜术制备。在电子显微镜下见到,SIF细胞成团分布在豚鼠腹腔神经节内,包围着局部有孔的毛细血管内皮以及周细胞。在这些细胞的胞质内含有不同形态的颗粒泡,在质膜上可见胞吐影像。这些形态特点表明,豚鼠腹腔神经节的SIF细胞可能主要以内分泌或旁分泌的方式行使功能。此外,在这个神经节内,见到一种有较强嗜锇性,含有较丰富线粒体的暗SIF细胞。酸性磷酸酶除了定位于SIF细胞的典型的溶酶体外,也呈现在一些SIF细胞内的颗粒上。这些酸性磷酸酶阳性颗粒的性质不明,有可能是颗粒泡内含物进行代谢更新的一种机构。

The celiac ganglia from 9 mature guinea pigs of both sexes were fixed in a mixture of 2%glutaraldehyde and 2.5%depolymerized paraformaldehyde.The SIF cells in cryostat sections were discerned out through their eatecholamine fluorescence induced by the fixative.These cells were processed through routine procedures for electron microscopy after or without incubation for acid phosphatase(AcP).The ultrastructure and AcP activity of these cells were observed under an H-600 electron microscope.The SIF cells were found to be present in clusters adjecent to fenestrated capillaries.The exocytosis of vesicular granules of SIF cells were seen on the surface of their cell membrane facing the capillaries.In addition,some dark SIF cells which were more osmiophilic and rich in mitochondria were discovered in this ganglion.These ultrastructural features of the SIF cells indicate that they may perform functions of an endocrine and/or paracrine nature.After one.hour incubation in a Gomori-typed medium at 37℃,some granules.besides the typical lysosomes display the AcP activity.Although the nature of the AcP-positive granules hasn’t been elucidated a speculation is laid on that the presence of the enzyme on these granules may play a role of regulation in replacing the intragranular contents.