大白鼠中缝背核的纤维联系

CONNECTIONS OF THE DORSAL RAPHE NUCLEUS IN THE ALBINO RAT

应用Nauta法和HRP逆行传递法研究了大鼠中缝背核的传出和传入联系。传出纤维可分为上行和下行两部分。上行纤维分为三组.1.背侧组:经中央管周围灰持上行,沿途止于中央灰质和第Ⅲ脑室室旁灰质。2.中间组:行于被盖部中缝和两侧网状结构内并终止于网状结构核团。3.腹侧组:行于被盖腹侧区和内侧前脑束以及暖昧带。分别终止于中脑网状结构、脚间核、黑质和被盖腹侧区。经内侧前脑束上行可达丘脑腹侧核、丘脑后核、束旁核、脚内核、下丘脑外侧核,隔内侧核和视前外侧核等。下行传出纤维经中缝两侧下行,沿途终止于中缝桥核、脑桥被盖网状结构,中缝大核、延髓巨细胞核、旁正中网状核,中缝隐核、中缝苍白核和橄榄内侧副核,三叉神经感觉核和三叉神经脊束核。下行纤维可继续下降行于脊髓背外侧索、腹侧索前正中裂两侧,终止于前角,侧角,后角RexedⅠ~Ⅴ层,以及中央管周围灰质。中缝背核的传入投射来自:1.第Ⅲ脑室室周灰质和室旁灰质、中脑导水管周围灰质、第Ⅳ脑室室周灰质。2.视前内侧核。3.下丘脑前核、下丘脑外侧核、下丘脑背侧核、视上核。4.黑质(包括密带和网带)以内侧份为主。5.蓝斑复合体。6.中脑网状结构。7.中缝中央上核、线形核、桥核和中缝大核。8.延髓网状结构外侧核的背内侧份。

The connections between the dorsal raphe nucleus and other nuclei in the CNS were studied with Nauta degeneration method and HRP retrograde transport method.After dorsal raphe nucleus had been lesioned the degenerating efferent fibers could be divided into dorsal,intermediate and ventral groups.Via the dorsal group the ascending fibers passed through the periaqueductal gray matter and terminated in the substantia grisea centralis,substantia grisea periventricularis,and substantia grisea paraventricularis of the ventricularis tertius.The intermediate group,lying in the raphe of tegmental and reticular formation,was projected to the mesencephalic reticular formation,substantia nigra bilaterally.The ventral group,passing through the area tegmentalis ventralis,medial forebrain bundle(MFB),zona inserta,was projected to the lateral hypothalamic region,thalamus and telencephalon.The descending projections mainly from the intermediate and ventral groups,could be traced in the lateral region of the periaqueductal gray.The degenerating fibers passed through the ventral aspect of tegmentum along both sides of the raphe,and finally continued to the lower brain stem,and the dorsal horn of spinal cord.In the lower brain stem,the descending degenerated fibers terminated in the nucleus raphe pontis,nucleus raphe magnus,nucleus raphe pallidus,nucleus raphe obscurus,nucleus reticularis tegmenti pontis,nucleus reticularis gigantocellulari,nucleus reticularis paramedianus,nucleus olivaris inferior accessoris medialis,and nucleus tractus spinalis nervi trigemini.After the injection of HRP into the dorsal raphe nucleus,labelled neurons were found in forebrain structures,consisting of nucleus periventricularis,nucleus anterior medialis thalami,nucleus habenularis lateralis,nucleus anterior hypothalami,nucleus dorsomedialis hypothalami,nucleus preopticus medialis,nucleus mamillaris lateralis,ventral tegmental area of Tasi,zona inserta,nucleus supraopticus,and nucleus interpeduncularis.Below the level of injection site,labeled neurons were found in substantia nigra(zona reticularis and compacta),periaqueductal gray,nucleus interpeduncularis,locus coeruleus,nucleus tegmenti dorsalis,nucleus centralis superior,nucleus raphe pontis,nucleus raphe magnus,and nucleus reticularis lateralis.