棉酚对人类精子作用的冷冻复型法超微结构研究

THE EFFECT OF GOSSYPOL ACETATE ON ULTRASTRUCTURE OF HUMAN SPERMATOZOA STUDIED BY FREEZE-REPLICA

应用冷冻复型透射电镜研究法,观察了连续口服醋酸棉酚50天后,人类精子超微结构的变化。发现除精子头尾的肥大、瘦小、萎缩、扭曲、断尾和结节隆起等一般改变外,精子内部、生物膜及膜内微粒皆有相应更敏感的变化。生物膜的变化系单位膜两叶片的相互贴附性有某些改变;膜内微粒的变化系表现特殊集结,即在头尾的质膜集结为窗格状,在后头区可集结为粒团;在后顶体膜则出现两类颗粒,其中小的一种表现为各向成行的阵列。核孔未发现变化。作者认为棉酚对精子的影响作用首先表现在生物膜及膜内微粒,而一般外形改变则是继发结果。文内还推论棉酚对精子影响的靶性关系,可能与精子质膜存在棉酚受体有关。

In order to elucidate the ultrastructural changes of human spermatozoa afteradministration of gossypol acetate 40 mg/day for 50 days,freeze-replica techniqueswere employed.In addition to the ordinary changes of spermatozoa,such as swelling,wasting,atrophy,tortuosity,nodulation in the head and/or tail region and fracture betweenhead and tail,the internal structure,biomembrane and intramembranous particles ofsperm also showed relatively more sensitive changes.The changes of biomembranerevealed certain shift in regard to the firmness of reciprocating attachment of inner-and outer-leaflets of the unit membrane.On the other hand,the changes of intra-membranous particles revealed a specialized arrangement,this is,on the plasmamembrane of head and tail they were concentrated into lattice work,whereas,in thehind-head region they accumulated as clusters.In the hind-acrosomal membranetwo types of particles,large and small,may be distinguished,the smaller ones werearranged orderly in lines of all directions.As for the nuclear pores,no changewas found in this study.It is considered that the influences of gossypol upon human spermatozoa,firstof all,were shown in the biomembrane and intramembranous particles,and theordinary changes in appearance were the secondary result.In the discussion,it’ssuggested that a special agent,gossypol receptor,responsible for the gossypol effectsto the sperm,may be present in the plasma membrane of the spermatozoa.