用显示乙酰胆碱酯酶的方法对兔耳动静脉吻合的初步观察

ARTERIO-VENOUS ANASTOMOSES (AVA) OF THE RABBIT’S EAR AS DEMONSTRATED BY THE METHOD FOR ACETYLCHOLINESTERASE

正常兔耳新鲜皮下组织铺片,用Karnovsky和Roots亚铁氰化铜法显示乙酰胆硷酯酶的方法可以显示兔耳皮下动静脉吻合(AVA)的动脉段和中间部的“胆硷能神经纤维”,后者呈致密网套状。被显示的AVA部分,形态清晰,结构完整,于显微镜下极易辨认。对262个AVA中间部的长度和外径进行了测量:最长者为620.6微米,最短者为81.9微米,平均长度为254.8微米;最大外径为109.2微米,最小外径为20微米,平均外径为52.2微米。作者认为测量AVA中间部较测量AVA全长更有意义。并依据形态特点对AVA进行了较细致的分类:简单型(包括细长式和粗短式)和复杂型(包括二分支式、三分支式、四分支式、复合式和环式)。本文对此法显示AVA与以往活体观察和用组织学方法研究AVA进行了比较,为进一步研究AVA提供了一种新方法。

Fresh subcutaneous tissue from the rabbit’s ear was processed with copper ferrocyanide method(Karnovsky and Roots,1964)for demonstrating acetylcholinesterase.The arterio-venous anastomoses were revealed by the abundant"cholinergic"nerve fibers present around its arterial and intermediate segments.These nerves formed a dense network around these segments of AVA which gives AVA a conspicuous and outstanding picture under the microscope.262 AVA were identified and their length and outer diameter of the intermediate segment were measured.The longest was 620.6μm,the shortest 81.9μm,and the averaging length was 254.8μm.Their outer diameters fell between 109.2~20μm,with an average of 52.2μm.The authors condered the measurements of the intermediate segment of the AVA give more significant information than the total length of the AVA.The AVA were classified into the following types according their morphological characteristics:the simple type(including the long and thin type,and short and thick type)and the complex type(including bifurcate,trifurcate,quarifurcate,compound,and circular types).This method of demonstrating AVA morphology was compared with former observations made directly in vivo or by histological methods and it serves as an new tool for the investigation of AVA.