电针对机体细胞免疫的影响

EFFECTS OF ELECTROACUPUNCTURE ON CELL-MEDIATED IMMUNITY OF THE HUMAN BODY

观察70例患者(包括60例针麻及10例药麻),在针(或药)麻前及诱导20分钟后各自患者耳垂取血一次,部分患者在针刺24小时后又取血一次。电针主穴为合谷或足三里。作为体外细胞免疫检测方法我们采用改进的微量全血技术做玫瑰花(包括活性、非活性)及淋巴细胞转化试验。在做活性玫瑰花试验的同时,计数白细胞总数及淋巴细胞分类百分数以计算活性玫瑰花形成细胞(RFC)的绝对值。结果见到在活性RFC中针刺诱导后提高均值为12.7±1.43,降低均值为6.8±1.77。活性RFC绝对值的提高均值为175.33±63.59。然而药麻组的活性RFC在药麻后变动不明显。在淋巴细胞转化试验中,提高均值为12.7±1.29,降低均值为7.0±2.19。在针刺24小时后仍见提高效应。以上提高变动多见于针前细胞免疫水平偏低或一般者,而降低变动可见于针前水平偏高者。在活性、非活性玫瑰花及淋巴细胞转化三项试验中,提高或降低变动相符,提高约12~13%,降低约6~7%,两者相比皆以提高为主。

70 patients including 60 cases under acupuncture anesthesia and 10 cases under drug anesthesia were observed.Before and 20 minutes after acupuncture the blood samples were taken from the patient ear lobes respectively,and in some patients taken once again 24 hrs after acupuncture.The electroacupuncture point Hegu or Zusanli was mainly adopted.As the method for detection of cell-mediated immunity(CMI)in vitro the improved microtechnique of whole blood for E-rosette(active and nonactive)and lymphocyte transformation tests was used.In performance of active rosetting the total leucocyte count and the differential lymphocyte count were done for calculation of absolute number of active rosette forming cells(RFC).The mean value of increase of active RFC was 12.7±1.43,the decrease was 6.8±1.77 after acupuncture.The increment of the absolute number of active RFC was 175±63.59.However no marked effect on the drug anesthesia group was found.In the lymphocyte transformation assay the increase was 12.7±1.49,the decrease was 7.0±2.19,and the enhancement effect still exhibited 24 hrs after acupuncture.In these tests an increase was mostly found in those with a lower or a usual CMI level;a decrease often found in those with a higher CMI level prior to acupuncture.The increase or decrease level in the results of three kinds of test(active,nonactive RFC and lymphocyte transformation)was similar,the increase range was 12~13%,the decrease range 6~7%.As the former compared with the latter,the promotion was prominent by all means.