醋酸棉酚对大鼠垂体促性腺细胞及睾丸间质细胞超微结构影响的观察

ULTRASTRUCTURAL OBSERVATIONS ON THE GONADOTROPHIC CELLS OF ADENOHYPOPHYSIS AND THE INTERSTITIAL CELLS OF TESTIS IN RATS FOLLOWING GOSSYPOL TREATMENT

本文对服棉酚后的大鼠垂体促性腺细胞和睾丸间质细胞进行了电镜观察。比较了短期和长期服药的效应。实验表明,在电镜下,垂体前叶促性腺细胞有四种类型。对照组动物以Ⅰ、Ⅱ型细胞为主。尤其以Ⅱ型细胞数量最多,占65%。服药组Ⅱ型细胞随着服药时间的加长而有递减趋势。Ⅲ型细胞在对照组动物为数极少,只有6%,而服药组则为主要类型。服药一个月的大鼠Ⅲ型细胞占促性腺细胞总数的56%。服药二个月的占44%,服药一年的占58%。Ⅳ型细胞胞浆内粗面内质网高度膨胀,形成大泡,几乎占据整个胞浆,形态上类似切除性腺后出现机能亢进的“阉割细胞”。这种细胞未见于对照组和服药一个月的动物,而于服药二个月后才开始出现,占促性腺细胞的13%。睾丸间质细胞的形态与对照组比较没有明显差异。最后讨论了这些变化的意义以及棉酚作用促于性腺细胞的可能机制。

Observations of the ultrastructural changes on the gonadotrophic cells of adenohypophysis and the interstitial cells of testis were carried out in rats following gossypol treatment.It was found that four types of gonadotrophs in the rat adenohypophysis could be classified under the electron microscopic observation.The contral groups mainly consisted of the typesⅠandⅡgonadotrophic cells and the latter appear to be the most numerous cell type,which constitute about 65%of the total gonadotrophs.Following gossypal administration,the number of typeⅡcells decreased obviously while that of the typeⅢcells increased steady and became the principal type of gonadotrophic cells in gossypol-treated animals.The proportion of the typeⅢcells were calculated as 56%,44%and 58%of the total gonadotrophs afterⅠ,2 and 12 months of gossypol treatment,respectively.The typeⅣcells were characterized by the appearance of dilated vesicles derived from rough endoplasmic reticulum which occupied almost the entire cytoplasm.Morphologically,these cells were similar to"castration cells"which appeared in the rat adenohypophysis after gonadectomy.They were observed in the experimental animal groups following two months of gossypol treatment,and their proportion was about 13%of the gonadotrophs.No significant ultrastructural changes in the interstitial cells of the treated rat testis could be observed.The significance of the above mentioned changes and the possible mechanism of gossypol effect on the gonadotrophs were discussed.