醋酸棉酚对人体精子的影响——精子的细胞学、细胞化学及电镜的观察

CYTOLOGICAL,CYTOCHEMICAL AND ULTRASTRUCTURAL OBSERVATIONS ON THE HUMAN SPERM FOLLOWING GOSSYPOL ADMINISTRATION

在城市及农村两组连续口服醋酸棉酚分别为15毫克/天和22.5毫克/天的不同时间后,分别取精液作细胞学、细胞化学及超微结构的观察。与服药前自身对照相比较,分析了棉酚的抗精子发生的作用效应。精子计数与细胞学分析结果表明:口服棉酚四周后畸形率增高者不多,而八周后在精子数目剧减的同时,涂片内脱落生精细胞(主要为精母细胞及精子细胞)明显增多,反映了棉酚作用部位主要为精子发生过程中的变态期精子细胞和中、晚期精母细胞。服用棉酚后可引起精子中段SDH活性减弱及线粒体超微结构的损伤(空泡化,排列紊乱到线粒体的全部消化)表明棉酚对精子能源代谢有一定的抑制作用,但对精子顶体PLE的影响则似为继发性性质。口服棉酚后可见精子核内染色质的超微结构产生异常变化,提示了棉酚影响精细胞核酸代谢的可能性。

The clinical trials of gossypol at the dosage of 15 mg and 22.5 mg per day were carried out in the city and the rural area respectively.The results of cytological,cytochemical as well as ultrastructural studies of the semen obtained before and during gossypol administration were as follows:1.It was shown that a decrease in sperm count and the occurrence of abnormal sperm in semen was detectable 4 weeks after gossypol administration.By the 8th week the sperm count being reduced markedly in semen,while exfoliated spermatogenic cells(including mainly spermatids and spermatocytes)increased concomitantly,the date indicated that the site of gossypol action was at the spermiogenic spermatids as well as the mid-and late stage spermatocytes.2.Decrease in the activity of SDH in the middle piece of the sperm and damage to the spiral mitochondrial sheath including derangement,vacuolation,and loss of mitochndrial cristae were observed following gossypol treatment.Such changes suggested that gossypol might suppress the energy matoblism of sperm.The effect of gosspol on the PLE of acrosome system appeared to be secondary.3.The ultrastructural changes of sperm nuclear chrmatin after gosspol treament suggested the possibility that gosspol might exert effect on the matoblism of the nuclic acid.