大白鼠视前区的传入性联系

AFFERENT CONNECTIONS OF THE PREOPTIC REGION IN THE RAT

应用辣根过氧化物酶轴突逆行传递的方法研究大白鼠视前区和下丘脑前核传入联系,采取了微量注射时多种途径入路,并结合离子透入的方法。HRP注射于视前内侧区,所有例子在下丘脑室周核、室旁核、视上核、下丘脑前核、下丘脑腹内侧核、下丘脑后核、蓝斑等都有标记神经细胞。终纹床核和中缝背核除个别例子外,也含有标记神经细胞。隔内侧核、外侧核和中脑水管周围灰质、脚间核仅少数例子中显现标记,且标记数量较少。HRP注射于视前内、外侧区之间。除上述HRP注射于视前内侧区标记核团以外,增加了丘脑旁带核、连接核、下丘脑外侧核。多数例子还增加了乳头体上核,个别例子在中央上核和皮质杏仁核以及内侧杏仁核内见到标记神经细胞。酶液扩散到伏隔核和斜角带后,增加伏隔核、嗅前核、个别例子还在中央上核,线形核尾侧,吻侧以及尾状核头的基底部见到标记。HRP注射于下丘脑前核,在隔外侧核、终纹床核、视前室周核、视前内侧核、室旁核、视上核、下丘脑腹内侧核、下丘脑外侧核、中脑水管周围灰质、蓝斑等观察到标记神经细胞,其中视上核及少数例子中室旁核均为双侧性标记。

The afferent connections of the preoptic region in the rat were studied with retrograde horseradish peroxidase(HRP)method.The mico-injection syringe needle or microelectrophoretic pipette was inserted vertically into the brain.In some cases,the injection was made with the needle inclined and directed contralaterally or in inclined direction through the cerebellum.The results were as follows:Following HRP injection into the medial preoptic area,labeled neurons were found in periventricular hypothalamic gray,paraventricular nucleus,anterior hypothalamic nucleus,supraoptic nucleus,ventromedial hypothalamic nucleus,posterior hypothalamic nucleus and locus coeruleus in all cases.In some cases,labeled cells were also observed within bed nucleus of stria terminalis,dorsal raphe nucleus.In addition,in a few cases,a small number of labeled cells could be found in lateral septal nucleus,medial septal nucleus,periaqueductal gray matter and interpeduncular nucleus.After injection into the region between the medial and lateral preoptic area,besides the above mentioned labeled nuclei,there were still a number of labeled cells found in paratenial nucleus,nucleus reuniens and lateral hypothalamic nucleus,in some cases also found in the supramammillar nucleus,and in one case even in the cortical and medial amygdalar nuclei.Following the injection of HRP into the anterior hypothalamic nucleus,retrograde labeling of neurons was observed within the lateral septal nucleus,preoptic periventricular nucleus,medial preoptic nucleus,supraoptic nucleus,periventricular hypothalamic nucleus,paraventricular nucleus,ventromedial hypothalamic nucleus,periaqueductal gray matter and locus coeruleus in all cases.Among them,supraoptic and paraventricular nuclei were labeled bilaterally in some cases.