大白鼠导水管周围灰质的传入性联系研究

AFFERENT CONNECTIONS OF THE PERIAQUEDUCTAL GRAY IN THE RAT

应用辣根过氧化物酶微量及离子透入法注射在大白鼠的中脑导水管周围灰质的背侧区、外侧区、腹外侧区以及间脑与中脑交界处第Ⅲ脑室室周灰质部位。在导水管周围灰质的背侧区注射后,标记细胞可在:室旁核、下丘脑腹内侧核、下丘脑背内侧核、下丘脑后核、乳头体内侧核、暖昧带、导水管周围灰质腹外侧核和外侧核、上丘、下丘、中缝背核、第Ⅳ脑室室周灰质中出现。在导水管周围灰质的外侧区注射后,标记细胞则可在终纹床核、室旁核、下丘脑前核、下丘脑腹内侧核、丘脑内侧核、后联合巨细胞间质核、暖味带、导水管周围灰质腹外侧核、黑质网带、中缝背核、第Ⅲ及第Ⅳ脑室室周灰质中出现。在导水管周围灰质的腹外侧区注射后,始终可有标记的核群是。视上核、室旁核、第Ⅲ脑室室周灰质、导水管周围灰质外侧核、黑质网带、中缝背核、蓝斑。在间脑与中脑交界处第Ⅲ脑室室周灰质注射后,有标记的核群是:终纹床核、视上核、室旁核、下丘脑腹内侧核、丘脑内侧核、暖昧带、导水管周围灰质外侧核、黑质网带、中缝背核和蓝斑。

The afferent connections to the periaqueductal gray in the rat were examined by means of retrograde axonal transport method using horseradish peroxidase(HRP).The microinjection syringe needle or micro-electrophoretic pipette was inserted stereotaxically by an oblique approach through the cerebellum.HRP was introduced into the dorsal,lateral,ventrolateral areas of periaqueductal gray or periventricular gray of ventriculusⅢat posterior commissure level respectively.The results were as follows:1.Injections of HRP in the dorsal area of periaqueductal gray(PAG).Numerous labeled cells were consistently found in the paraventricular nucleus,dorsomedial and ventromedial hypothalamic nuclei,posterior hypothalamic nucleus,medial mamillary nucleus,zona incerta,lateral and ventral nuclei of periaqueductal gray,superior colliculus,inferior colliculus,dorsal raphe nucleus and periventricular gray of ventriculusⅣ.In some cases,a smaller number of cells was found in the zona reticulata of the substantia nigra,and lateral hypothalamic nucleus.In one case,the enzyme was injected into the dorsal area of PAG,which diffused to the superior and inferior colliculi,resulted in labeling of Forel’s field and reticular nucleus of thalamus.2.Injection of HRP in the lateral area of PAG.HRP-positive cells were observed within the bed nucleus of the stria terminalis,paraventricular nucleus,anterior hypothalamic nucleus,ventro-medial hypothalamic nucleus,medial thalamic nucleus,nucleus interstitialis magnocellularis,commissural posteriors,zona incerta,ventral nucleus of PAG,zona reticulata of substantia nigra,formatio reticularis of mesencephalon,dorsal raphe nucleus,and periventricular gray of ventriculusⅢandⅣ.In some cases,a small number of cells was found in the supraoptic nucleus,superior and inferior colliculi.3.Injection of HRP in the ventrolateral area of PAG.In all cases the greatest number of HRP-positive neurons was shown in the supraoptic nucleus,paraventricular nucleus,periventricular gray of ventriculusⅢ,lateral nucleus of PAG,zona reticulata of substantia nigra,dorsal raphe nucleus and locus coeruleus.The labeled cells were mainly located ipsilaterally.But the labeling of supraoptic nucleus was observed bilaterally.In one case the enzyme diffused to the reticular formation,a smaller number of cells was found in nucleus cuneifomis and mesencephalic reticular formation of more caudal position.4.Injection of HRP in the periventricular gray of ventriculusⅢ.Following the injections of HRP in the periventricular gray of ventriculusⅢ,numerous HRP-positive cells were consistently observed in the bed nucleus of the stria terminalis,supraoptic nucleus,paraventricular nucleus,ventromedial nucleus of hypothalamus,medial nucleus pars medialis of thalamus,zona incerta,ventral nucleus of PAG,zona reticulata of substantia nigra,dorsal raphe nucleus and locus coeruleus.