从临床细胞学的观点探讨人肺鳞癌细胞系(LTEP-78)的细胞形态学特征

STUDIES ON MORPHOLOGY OF LONG-TERM CULTURED PULMONARY SQUAMOUS CARCINOMA CELL LINE(LTEP-78)IN VIEW OF CLINICAL CYTOLOGY

本文报告了从临床细胞学观点对长期体外培养的人肺癌细胞进行形态学研究的结果。发现虽然经长达一年半的体外培养,人肺鳞癌细胞系仍保留有鳞癌细胞临床诊断的标准特点。大多数细胞仍为圆形或多边形;边界清楚;核位于细胞中央;胞浆匀实;并出现典型的鳞癌细胞,如圆形癌细胞、蝌蚪形癌细胞及多核癌细胞等。在培养细胞中也出现细胞分化状态的改变,如少数细胞保存着低分化鳞癌细胞的状态,甚至呈未分化的恶性裸核,而大多数细胞转化为中分化的鳞癌细胞。值得注意的是,在少数培养细胞中出现腺癌细胞样的改变,如癌细胞的胞浆内出现单个或多个大空泡,并将核推到一边。有的细胞呈典型的恶性“印戒细胞”。因此,我们认为体外培养的人癌细胞仍可反映出人体癌细胞的临床诊断特点,所出现的差异可能是体外环境选择而使细胞改变的结果。

Morphology of cells of long-term cultured pulmonary squamous carcinoma cell line(LTEP-78)and cancer cells taken from sputa of fifty lung cancer patients was studied in view of clinical cytology.The results showed that the long-term cultured cell line still have had the characteristics of squamous cell carcinoma,such as round or squamous cell outline,definite cellular border,evenness in density of cytoplasm,centrally placed nuclei and so on.On the other hand,it appeared that they have undergone some changes which resembled to adenocarcinoma cells,such as malignent signet-ring cell.We concluded,therefore,that the long-term cultured cancer cells could still keep the characteristics as cancer cells taken freshly from lung cancer patients.The differences from the fresh lung cancer cells found in cultured cells could be explained by the effects of in vitro circumstance which is quite different from that of the human body.