大白鼠肝癌细胞株FSK 7901,FSK 7902的建立和特点

ESTABLISHMENT AND CHARACTERISTICS OF THE HEPATOMIC CELL LINES FSK 7901,FSK 7902 OF THE RAT

用DEN诱发大白鼠的肝癌,取其肝癌结节,采用组织培养法建成肝癌细胞株FSK 7901,FSK 7902。二株肝癌细胞染色体数目的主流范围在63~84,主流数在81。FSK 7901株的主要特点是细胞排列紧密,小型肝癌细胞,分布于肝癌组织区内或边缘且有一定的规律。核旁嗜酸性区域明显,含有肾形畸形核的肝癌细胞较多。核仁的体积大而数量少。细胞数目的增加比FSK 7902慢。FSK 7902株的主要特点是肝癌细胞排列疏松,小型肝癌细胞的分布没有规律,核旁嗜酸性区域比FSK 7901株内的少且不明显。含有圆形核的肝癌细胞多,即细胞核的畸形程度,没有FSK 7901株内的严重。核仁体积比FSK 7901株内的小,但数量多。细胞数目的增加比FSK 7901快。

Epitheloid hepatomic cell lines FSK 7901,FSK 7902 were established in cell culture from male rats with an average body weight 103.03±26.73 g sacrified 131±24.94 days after feeding with 107.1±25.65 mg.of diethylnitrosamine(DEN).The chromosome number of the majority of the cells from FSK 7901,FSK 7902 falls between a range of 63~84 with a mode of 81.The cells are very closely arranged in FSK 7901,but loosely in FSK 7902.The distribution of the smallest basophilic hepatomic cells of FSK 7901 are regularly around or within the new growth area,but in FSK 7902 are irregularly scattered.The acentrically displaced bean-shaped nuclei of moderate or large hepatomic cells with large juxtanuclear acidophilic area are more numerous and distinct in FSK 7901 than in FSK 7902.The growth of FSK 7902 is more rapid than FSK 7901 as judged from the main cell cycle time from population doubling time during lorgarthmic growth of culture and the average of mitotic index.