中国地鼠(Cricetulus griseus)染色体的三种带型

THE BANDING PATTERNS OF WILD CHINESE HAMSTER(CRICETULUS GRISEUS)CHROMOSOMES

中国地鼠是产于我国北方的应用广泛的实验动物,早年由我国学者发现它对黑热病(LD小体)敏感^([1,2])。由此引起人们对这种动物的兴趣并试图进行人工繁殖^([3])。后来Yerganian^([4])花了较长时间把这种动物繁殖成纯系。伴随组织培养技术的发展,发现这种动物有染色体数少,细胞株易于获得,增殖传代快,二倍体细胞株稳定等优点,而被细胞遗传学和细胞生物学工作者广为利用。在六十年代初Bender和Gooch^([5])用这种动物进行了放射线与染色体畸变效应的研究,Hampar和Ellison^([6])用单纯疱疹病毒感染这种动物,研究了染色体的畸变。

The G and C banding patterns and the silver stained NOR’s of the chromosomes of wild Chinese hamster’s(Gricetulus griseus)bone marrow cells were studied,and des-cribed.In G-banding,a heterochromatin band was shown to be present on the long arm of chromosome No.1 at a distance of about 1/4 the length of the long arm from the centro-meres.This band was also seen in some of the karyotypes published in the literature but has not been described before.Another hitherto undescribed heterochromatin band was found on the short arm of chromosome No.2.Silver-stained NOR’s were not only seen on chromosomes Nos4,5,6,7,8,9 and 10 as in laboratory raised Chinese hamsters but also on chromosome No.3 with a frequency of 79.6%.None was found on the X-chromosome.The results were discussed.