棉酚对男性精子影响的电子显微镜观察

ELECTRON MICROSCOPIC OBSERVATIONS OF GOSSYPOL EFFECTS ON THE SPERMATOZOA OF HEALTHY MEN

本研究报道育龄男子服醋酸棉酚后不同时期精液中精子超微结构改变的观察结果。育龄男性志愿者每日服药剂量为20毫克,连服50天后,改服每周40毫克的维持量,共观察了服药前5例作为自身对照,他们于服药后30天和50天分别再予检查;另对服维持量第84天的7例进行了观察。精液离心之沉渣用戊二醛-锇酸固定,丙酮脱水,Epon 812包埋,电镜观察。观察结果表明,螺旋鞘膜线粒体和顶体-核帽系统是对棉酚最敏感的细胞器,出现损伤最早、最严重,轴丝体和细胞核亦有不同程度的改变。严重的可见整个精子崩溃解体,精液中出现细胞残体和大量脱落细胞。根据上述结果,对棉酚造成精子损伤的性质及其意义予以分析和讨论。

The ultrastructural changes in spermatozoa of men were studied at various time intervals:following gossypol treatment.Fertile-age male volunteer were given a daily dose of 20 mg of gossypol acetate for 50 days,and followed by a maintenance dose of 40 mg per week.Semen examinations were made on five subjects before treatment as self-control and on the 30th and 50th day respectively after gossypol treatment.Another 7 cases taking maintenance dose were examined on the 84th day after treatment.The precipitates of semen following centrifugation were fixed in glutaraldehyde-osmic acid.After dehydration with graded acetone,the specimens were embedded in Epon 812 and observed in a JEM electron microscope.It was shown that the spiral sheath of mitochondria and the acrosome head cap systems were the most sensitive organelles responding to gossypol.They were the first to show a detectable damage and suffered the most severe damage.The axial filaments and nuclei also demonstrated different degrees of ultrastructural changes.The severely damaged spermatozoa finally disintegrated.Appearance of cell debris and exfoliated unmatured spermatogenic cell in semens were evident.Based on the above results,the nature and significance of the ultrastractural damages of spermatozoa induced by gossypol were analysed and discussed.