疼痛对垂体前叶超微结构的影响

EFFECT OF PAIN ON THE ULTRASTRUCTURE OF ANTERIOR LOBE IN PITUITARY

应用免疫荧光抗体法或免疫酶组化法(酶-抗酶复合体,简称PAP法)发现某些哺乳类动物(牛、羊、猪和鼠)及人垂体前叶的促肾上腺皮质激素(ACTH)细胞,可分泌AGTH、β-内啡肽、β-MSH及β-LPH等激素;并且这些激素被证明定位在该细胞的同一分泌颗粒内^([1,2]),在应激状态下,这些激素是同时被分泌出,并排出量增多^([3])。在暴冷或手术时,血清中的皮质酮、生长激素(GH)及催乳素(PRL)的浓度均增高。

Our experiments were designed to observe the cytological changes of the anterior lobes in rats pituitary at electron microscopic level after electric pain-stimulation.obvious changes were founded in the ACTH,GH and PRL cells and slight changes in the FSH and TSH cells.However,the LH cells remained to resemble the normal cells.In the experiment group of the somatic painful stimulation the results were as follows:The ACTH cells were irregularly shaped,with extended thin and long plasmatic processes which interdigitated with GH cells.The processes filled with secretory granules.Some of the Secretory granules aligning along the plasmatic membrane showed pale density.The flat cisternae of rough endoplasmic reticulum(RER)appeared to be slightly dilated.The sizes of the bodies of GH and PRL cells increased.In the GH cells there were numera media cisternae in RER,their Golgi complex was well developed,and the secretory granules were distributed to the periphery of the cell bodies.All these phenomenous showed the secretory activity of these cells was enhanced.The PRL cells contained well developed Golgi complex and abundant RER.In the experiment group of the visceral painful stimulation the results were as follows:There were more striking changes of parenchymal cells of anterior lobes in rats pituitary than those of the experimental group of the somatic painful stimulation.The cell bodies of ACTH,GH and PRL cells showed hypertrophy,the nucleus enlarged and nucleolus were relatively prominent.The dense granules decreased in number and the pale or empty visicles almost situated near the plasmic membrane in ACTH cells.In the GH cells large vacuoles were distributed throughout the cytoplasma.The number and density of the secretory granules decreased.In PRL cells the Golgi complex were extended,the dictyosomes increased,in addition,RER,polyribosomes and mitochandria were more aboundant than that in these normal cells.These cytological changes suggested secretory and synthetic activity of these cells were enhanced.At last,pain in relation to several hormones such as β-endorphin,β-LPH,ACTH and β-MSH etc.were discussed by the author.