棉酚对大鼠毒性的电子显微镜观察

ULTRASTRUCTURAL OBSERVATION OF THE TOXIC EFFECT OF GOSSYPOL ON RATS

棉酚对雄性大鼠有明显的抗生育作用^([1,2])。用电镜和同位素放射自显术对服药后大鼠睾丸变化动态的观察表明棉酚主要破坏生精上皮的精子发生过程,其中变态期精子细胞和中、后期初级精母细胞对棉酚的毒性最为敏感^([1])。4000多名志愿服药者的临床试验进一步证明了棉酚的抗生育效果肯定可靠^([3])。精液脱落细胞的分析亦以精子细胞和精母细胞的脱落为主^([4])。棉酚对生精细胞的这种毒性作用,为当前寻找男用避孕药提供了前景。棉酚对其他脏器组织的毒性作用亦已有报道。

The present study was designed to examine the toxic effect of gossypol on the heart,liver,kidney,adrenal and testis of the Wistar rats with the electron microscope.Gossypol was administered orally at a daily dose of 30mg/kg body weight for 1-3 weeks,the tissues were secured at various time intervals and prepared for observation.It was shown that no significant ultrastructural changes could be observed in the heart muscle,kidney,and adrenal of animals fed on gossypol at the dosage that had damaged to testis.The latter demonstrated obvious degenerative changes in spermatids and spermatocytes 3 weeks following treatment and appeared to be more sensitive to the effect of gossypol poisoning.Gossypol induced distension of the endoplasmic reticulum and caused an increases in amount of smooth endoplasmic reticulum as well as lysosomes of the hepatic cells.Other subtle ultrastructural changes in the hepatic cells including the decrease of glycogen granules,displacement of ribosomes from rough endoplasmic reticulum and the distension of canaliculi biliferi were also discernible.Based on the above results,the toxic efficacy of gossypol was analysed and discussed.