细胞松弛菌素D对ECa-109细胞的作用

ACTION OF CYTOCHALASIN D ON CELLS OF ESTABLISHED HUMAN ESOPHAGEAL CANCER LINE

细胞松弛菌素(Cytochalasin)是一类真菌的代谢产物。自Carter 1967年首次报道以来^([1]),迄今已发现有10余种。细胞松弛菌素具有广泛的细胞生物效应。诸如能专一性地作用于哺乳类动物细胞微丝系统,影响细胞的运动^([2~5])。抑制细胞质的分裂而不影响核的分裂,致使形成双核及多核细胞^([1,6])。此外,它还可以引起细胞产生排核现象^([7,8]),调节质膜与植物凝集素的反应^([9])等。晚近,不少实验室又把细胞松弛菌素与离心术结合起来,获得去核细胞及小细胞^([10,11])。

The effects of cytochalasin D(CD),isolated from Engleromyces goetzii found in our country,on cells of established human esophageal cancer line(ECa-109 cell line)were investigated.It was demonstrated that CD was capable of inhibiting the growth of the cells at the concentration of 0.2μg/ml as indicted by growth curve.The action of CD,however,is reversible,and the cells will gradually grow again normally following the withdrawal of CD.The result also indicated that some proportion of the cells became binucleated or multinucleated after CD treatment.And as the duration of exposure time to CD increased.the multinucleated cells increased in number also.At the concentration of 0.5μg/ml of CD,a phenomenon of extrution of nucleus can be seen among the cells.The nucleus is departed from the cell body but still linked by a fine cytoplasm bridge(Fig.2).The CD-treated cells bacame enucleated when they subjected to centrifugation following CD treatment.The minicells obtained from enucleation appeared to be normal morphologically with nucleoli but retained only a little cytoplasm around the nucleus.Electromicroscopic observation showed that mitochondria and tonofibrils increased after CD treatment,but we failed to find any changes of microfilaments within the cells.