捶死及钙杀死两种杀死方法对家兔左心室正常糖原及异常糖原分布的影响

THE INFLUENCES OF METHOD OF SACRIFICING THE ANIMALS MEDULLA-SHOCK AND CALCIUM-BLOCK ON THE TOPOGRAPHIC DISTRIBUTION OF NORMAL AND ABNORMAL GLYCOGEN INRABBIT’S LEFT VENTRICLE

作者应用肾上腺素产生实验性心肌病变。从钙杀死及捶死的正常及实验的家兔心肌PAS及H.E染片的观察中,作者得出下列几点结论:1,钙杀死的动物较之捶死动物的心肌含有更多的糖原。2,钙杀死动物心肌内层与外层糖原含量相等而其功能皆为缩短心室纵径;司心室横径收缩的心肌中层糖原较少。因此作者认为心肌各层糖原含量与其功能有关。3,乏氧对心肌糖原的影响以外层最显著,心肌中层的血窦间地区(不包括血窦旁地区)糖原消耗较多,而乏氧对心肌内层亦有一定影响。作者认为以上差别是与其血液来源相关。4,在捶死动物中,血窦旁及乳头肌两侧以及心肌内层皆保留糖原一事再次证明糖原与血液供应的关系。5,心肌异常糖原不受乏氧影响,它可能是与蛋白貭结合的糖原。6,5—14天的病灶周旁异常糖原较多。14天以上的病灶结瘢修复,其周旁纤维康复,无异常糖原。在5天以内的材料中,病灶尚未形成,病变纤维周旁的纤维因受损与恢复程度不同故而糖原分布极不恒定。

A total of 23 adult rabbits were used in this study.Six were used as control andthe remaining 17 rabbits were injected with adrenaline to produce cardiac lesion.Theseanimals were sacrificed alternatively either by medullary-shock or clacium block after 1,3,5,7,10,14 and 21 days of experiment.The left ventricle was excised and fixed inCarnoy fixative,embedded in paraffin,stained with either H-E or PAS(McManus)method.Slides of saliva digestion and PAS staining were used for the identificationof glycogen.The following points of conclusion were drawn:1.More glycogen was present in the left ventricle of animals killed by calciumblock than those killed by medulla-shock.2.The glycogen content in the outer and inner myocardial layers of the normalanimal killed by calcium block was identical and that of the middle layer was lesser.Since these myocardial layers perform different functions in ventricular contraction,theauthors suggest that the glycogen content of the different normal myocardial layers wasassociated with its function.3.Medulla-shock causes anoxia of the heart.There were severe exhaustion ofglycogen in the outer layer during anoxia.The influence of anoxia was restricted to theintersinusal region(the parasinusal region was less influenced)of the middle layer.Theinfluence of anoxia to the inner layer was minimal.These phenomena were interpretedas due to the different blood sources of each layer.4.In the myocardium of the shocked animals,glycogen was constantly stagnatedat the following places:the perisinusal fibers of the middle layer,the lateral boundariesof the papillary muscle,and the inner layer of the myocardium.These phenomena fur-ther proved the relationship between the glycogen distribution and the blood supply.5.The perilesion deposition of glycogen due to adrenaline was not influenced bythe process of anoxia in any layer of the myocardium.The abnormal glycogen disap-peared coincidentally with other cytoplasmic material during degeneration.So,it wasbelieved that this glycogen probably was combined with the protein.The range ofabnormal glycogen distribution was in accordance with the severity of the lesion.6.Within the first 5 days of our experiment,the focal lesion had not yet form-ed,the injury of individual fiber was variable and so did glycogen distribution.Afterthe 14th experimental day,the lesion was replaced by scar tissue and the perilesion car-diac fibers reappeared to normal.Thus,there was no abnormal glycogen deposit in thatregion.Only between the 5th and 14th experimental days,the abnormal glycogen de-position was distinct.