创伤后大白鼠小脑皮质普金野细胞的有丝分裂

MITOTIC DIVISION OF PURKINJE CELLS IN THE INJURED CEREBELLAR CORTEX OF ADULT ALBINO RATS

最近几年来,已有不少文献报告,成年哺乳动物在实验性损伤之后,发现中枢神经系统大脑皮质神经元的有丝分裂,如徐静(1958[1],1959[2],1962[3])和АфанасьевЮ.Н.иКотовскийЕ.Ф.(1959[4])在大白鼠;雍克昌,陆德裕,雍慧仪(1959[5],1960[6],1960[7])在家兔和家猫。

Fifty-six adult albino rats weighing between 150--200 gm were used for the experi-ments.After resection of the interparietal bone,a portion of cortical tissues was remo-ved from the central lobe of the cerebellum.To some of them a piece of cotton wasleft at the wound serving as a stimulator.The animals were sacrified 2,3,4,5,6,and 7days after operation and specimens of the cerebellar tissues were removed from the woun-ded area and fixed in Carnoy’s fluid.Paraffin serial sections 6μthick were stainedwith hemztoxylin-eosin.For RNA of Nissl bodies and DNA of chromosomes,bothKorson’s toluidine blue-methyl green-orange G and Brachet’s pyronin-methyl green me-thods were used.3--5 days or 3--6 days(with cotton)after operation,prophase,me-taphase and anaphase of Purkinje cells in mitosis were found,among which metaphasewas the most numerous.It was suggested that the cotton,acting as foreign body,couldstimulate mitotic activity when it was applied to the wound.Basing on the location,shape and size of the cells undergo divisions,it leaves nodoubt that they are none other than Purkinje cells in mitosis.