骨软骨肉瘤单细胞纯株(515株)的建立

THE ESTABLISHMENT OF CLONE 515 OF THE STRAIN MEO OF OSTEOCHONDROSARCOMA

近年以来,哺乳动物细胞的离体培养,愈来愈多地引起形态、生化和生理各个不同方面研究者的注意。由于高等动物体内组织器官相互作用的复杂条件,细胞的某些特性很难于进行研究,而在离体培养的条件下,这些研究便成为可能。但是,通常认为形态上一致的一个细胞株,毕竟是由混杂存在于原始移植块的多种细胞生长而成。

The authors described the procedures for the establishment of clone 515 of the cell strain of osteochondrosarcoma by capillary method.A single cell was sucked into a capillary tube by a microsyringe under the control of a micromanipulator.A segment of the capillary tube with the cell was then planted into the plasma clot in a Carrel flask.After the addition of conditioned medium,the flask was incubated at 37℃.Daily ob-servations were made.The propagation of the single cell,the migration of its progenies out of the capillary tube and the change in form and size of the cells were recorded.63 days after the isolation,the progenies covered the whole bottom of the flask.First subculture was then made and the clone was established therefrom.