皮貭創伤組織的神經細胞分裂和細胞化学——1.神經細胞有丝分裂和糖元含量的关系

CELL DIVISION AND CYTOCHEMISTRY OF NERVE CELLS IN THE INJURED CORTICAL TISSUE Ⅰ.THE RELATION BETWEEN MITOSIS AND GLYCOGEN CONTENT OF THE NERVE CELLS

近几年来,越来越多的事实証明,高等动物成年有机体的神經細胞在某些条件下具有分裂和繁殖的能力。这些材料都有力的否定了很久以前的結論,认为神經細胞是高度分化的組織,和有机体的其他組織不同,它們在胚胎期的成神經細胞阶段就已經失去了分裂和繁殖的能力。

Portions of the cerebral cortices were removed under ether anesthesia and antiseptic conditions from the brains of 150 adult rats,weighing between 130—150 gm.and of the age between 4—5 months.The animals were killed by breaking their necks after 1,2,3,4,5,7,10 and 15 days,and specimens of brain tissue were removed from the wounded area and fixed within one minute in Shabadash’s fixing fluid.Paraffin sections of 6μin thickness were prepared as usual and stained by the periodic acid-Shift’s(PAS)procedure.Pretreatment with diastase served as the control.Mitosis of the nerve cells was observed between 3—5 days after the operation in the undamaged brain tissue near the wound.In the locality where the mitosis of nerve cells was found,a large amount of glycogen granules appeared in the matrix between cells,the walls of blood vessels,neutrophils,phagocytes,neuroglia and some of the nerve cells.The glycogen granules decreased in amount after 5—10 days.On the 15th day the glycogen granules disappeared in most of the brain tissues except occasionally a few were found around the blood vessels and the superficial layer of the cerebrum which resembled the conditions in the normal brain.The simultaneous occurrence of the mitotic division of the nerve cells and the increase of glycogen content of the brain tissue near the wound leads the author to con-sider that glycogen may serve as the rich enery source for cell division.