动情周期中小白鼠生殖系统的组织化学观察Ⅱ.子宫

HISTOCHEMICAL OBSERVATIONS ON THE FEMALE REPRODUCTIVE ORGANS IN THE MOUSE AT DIFFERENT STAGES OF THE ESTROUS CYCLE Ⅱ.UTERUS

序言子宫在动情周期中的变化极为显著,因此对于它在动情周期中的组织学及组织化学的变化以及性腺激素对于它的化学物质的影响,做过不少观察,积累了丰富的知识,但也产生了不少分歧的及未解决的问题。(一)核酸Stein[1]及Drasher[2-3]用化学分析的方法,测定子宫的核蛋白的含量在动情激素发生作用的时期增加。

The uterus of the virgin albino mouse,aged 2—3 months,was observed microscopically atvrious stages of the estrous cycle.The techniques used for the demonstration of ribonucleic acid(RNA),desoxyribonucleic acid(DNA),glycogen,mucous substance,alkaline and acid phosphatasesand lipoid substances were the same as my previous report on the histochemical observations of themouse vagina.RNA in the epithelial lining,uterine gland,connective cells and muscle cells was increased inthe proestrus and estrus and decreased in the metaestrus and dioestrus.The epithelial cells gavemore pronounced changes.DNA in the mouse uterus also gave cyclic changes.The Feulgenreaction of the epithelial nuclei were weak in the late metaestrus and dioestrus and intensifiedin the proestrus and estrus.These nuclei were large in size and filled with coarse chromatin gra-nules.In the early metaestrus,the nuclei were smaller and narrower and filled with dense andfine chromatin granules.In the early metaestrus,and particularly in the late metaestrus,Feulgenpositive granules of different sizes appeared in tht cytoplasm of the lining epithelium.These cy-toplasmic chromatin granules were considered to be disintegrated nuclear material.The glycogen in the uterus was very conspicuous in the late metaestrus mainly due to the infil-tration of the glycogen bearing white blood corpuscles.In tht proestrus and estrus,glycogen granuleswere found in the cytoplasm of the epithelial and connective tissue cells but not in the white blood cor-puscles.Muscle glycogen was more conspicuous in the proestrus and estrus.The uterine epithelium began to show mucous reaction in the proestrus and became strong inthe estrus.Mucous substance was found in the lumen instead of the epithelium in the earlymetaestrus.The activity of the alkaline and acid phosphatases in the uterine epithelium was weak in thedioestrus,stronger in the proestrus and maximal in the estrus and early metaestrus.Thelongitudinal muscle manifested stronger activity than the circular layer,but the former failed togive evident cyclic change while the latter showed a similar cyclic changes as that of the epitheLium.The lipoid substances in the uterine epithelium was smallest in amount in the proestrus andestrus and greatest in the metaestrus and dioestrus.The lipoid granules were mainly located inthe subnuclear region of the surface epithelium.