哺乳动物唾液腺的结构及其细胞染色性质的初步观察Ⅰ.腮腺的观察

THE STRUCTURE OF MAMMALIAN SALIVARY GLANDS AND THE STAINING PROPERTIES OF THEIR CELLS Ⅰ. PAROTID GLAND

1.本文叙述了22种哺乳动物(包括7个月)腮腺的构造,并对腮腺细胞的“黏液性”和核外染色性做了一些初步的组织化学观察。2.大部分动物(除去牛、羊和豚鼠)的腺泡细胞都含有PAS阳性的“黏液样颗粒”,其染色性质与典型的黏液细胞不同,前者呈β-异色性,后者虽亦为PAS阳性,但呈恒定的γ-异色性。3.人、骡和齿类腮腺腺泡细胞的核外染色质较多,在食肉类很少,在有的动物如刺猬等几乎看不出。4.有些动物的闰管及分泌管细胞表面有PAS阳性结构,食肉类分泌管细胞顶部含PAS阳性细粒,从染色性质上看来可能是黏液性质。

The structure of the parotid glands of 22 animals,representing 7 mamma-lian orders,was briefly described and some histochemical characteristics of their cells were studied.The tissues were fixed in Helly’s and Carnoy’s fluids,em-bedded in paraffin and stained with McManus PAS method and Mucicarmine for mucous substance,methyl green-pyronin and gallocyanin for chromidial substance and toluidine blue for metachromasia.Millon’s reagent was used for the identification of protein.In most of the material studied,the acinar cells with PAS positive granu-les were shown to beβ-metachromasia with toluidine blue.The staining reac-tions of these granules differed from that of typical-mucous cells in that the latter,although PAS positive,showedα-instead ofβ-metachromasia.In the acinar cells of rodents,men and mules,the chromidial substance was relatively abundant,while in carnivores and others it was negligible in amount.In still others mere traces were found.PAS positive substance was present on the surface or in the apical cytoplasm of the intercalary and salivary duct cells in many animals.It was not glycogen since it resisted diastase digestion.Its phy-siological signifieance awaits further elucidation.The intercalary duct of ham-ster contained PAS positive fine granules which could not be stained by muci-carmine and showed more intense Millon reaction than that of acinar cells.The salivary duct of moles had special epithelium which contained distinct spherical granules that stained pink with the PAS method.