疲劳对于小白鼠肝脏的糖元及核糖核酸的影响

EFFECTS OF FATIGUE ON THE DISTRIBUTION OF GLY-COGEN AND RIBONUCLEIC ACID IN THE MOUSE LIVER

1.本實驗係用44個體重15-25克雄性小白鼠,22個用電刺激使牠們在木匣裏奔跑1-4小時,觀察全身在緊張運動後肝臟糖元與核糖核酸的變化。以11個飢餓和11個正常動物作對照。2.小白鼠肝糖元經過1小時的飢餓即開始減少,2-31/2小時大約減少一半,到6小時的時候只有少數細胞還有少量的糖元顆粒,或完全消失。核糖核酸變化較慢,1小時的飢餓無顯著變化,2-6小時開始分解,由大塊的變成小塊、桿狀或絲狀,分佈也比較分散,核及核仁不顯變化,個別例子的靜脈竇有擴大現象。3.疲勞動物的糖元及核糖核酸的變化比飢餓動物大得多,大多數疲勞2-31/2小時的標本顯示糖元顆粒已全部消失,1小時的也只有少數細胞尚含有少量糖元。緊張運動後的核糖核酸也有分解和減少現象,核内染色質聚成團塊或貼附在核膜内側,中央靜脈和靜脈竇顯著擴大充血,肝細胞縮小,Kupffer氏細胞增多核染色加深。

Under stimulation of electric current adult albino mice were made to run around on the wire without stop for 1 to 4 hours in a wooden case.In starved animals glycogen began to decrease 60 minutes after depri-vation of food and reached to about half within 2 to 31/2 hours.Six hours later only a few liver cells still contained some glycogen granules.Deple-tion of ribonueleic acid took place at a much slower rate.It broke from larger masses into tiny rods and filaments,sparsely distributed.No change of nucleus and nucleolus was noted.In exhausted animals changes of liver glycogen and ribonucleic acid were much more conspicuous than the starved ones.In most cases 2 to 31/2 hours’fatigue caused complete disappearance of glycogen granules and the reduc-tion of the ribonucleic acid.The chromatin condensed into larger knots or adhered to the inner side of the nuclear membrane.Central veins and sinusoids became enlarged and congested.Liver cells became smaller.Kupffer cells increased in number and their nuclei became darker.