小白鼠长骨生长的组织学观察

HISTOLOGICAL OBSERVATIONS ON THE GROWTH OF LONG BONES IN THE MICE

关于长骨长度的增长,很久已经知道是在骨端,而且两端的增长并不一致。许多学者的观察都证明其生长率大的一端在肱骨、胫骨和腓骨的近侧,股骨、桡骨和尺骨的远侧;而生长率小的一端在胧骨、胫骨和腓骨的远侧,股骨、桡骨和尺骨的近侧。

The long bones of the fore and hind limbs of 1-day and 5-day new born mice werefixed in Zenker’s 10%neutral formalin and Carnoy’s fixatives.They were sectionedwithout decalcification and stained in Mallory for general structure,in McLean&Bloom’smodification of Kossa for calcification and in periodic acid-Schiff’s reagent for glycogenrespectively.It was found that in the humerus the length of the cartilaginous cell columns,thedegree of calcification in the cartilaginous matrix and the extension of the subperiostealcollar in the cartilaginous level were all more extensive in the proximal end than that of thedistal.The situation was the opposite in the case of femurus.The development in theproximal ends of the radius and ulna were less extensive than the distal.On the otherhand,the proximal ends of the tibia and fibula were more extensive than the distal.Hence,the increase in length of the fore limb would be mainly in the shoulder and wrist jointswhile that of the hind limb in the knee joint.The distribution of glycogen in the chondrocytes was found to be more abundantin the transitional zone between the flattened and hypertrophied cell columns.Thesignificance of this distribution in relation to phophatases,nutrition and calcification wasdiscussed.