绝食对小白鼠肝脏磷酸酶糖元及核糖核酸在分布上的影响

INFLUENCE OF STARVATION ON THE DISTRIBUTION OF PHOSPHATASES,GLYCOGEN AND RIBONUCLEIC ACID IN THE MOUSE LIVER

1.本实验系用年幼的小白鼠绝食66至120小时,及重喂11小时至3日,观察肝脏内硷性及酸性磷酸酶、核糖核酸及糖元的改变。结果绝食的肝脏内糖元及核糖核酸完全消失;硷性磷酸酶中等量增加;酸性磷酸酶无变化。重喂的肝脏内糖元比对照标本更多,核糖核酸未完全恢复,硷性及酸性磷酸酶变化不一致。2.绝食66小时以上肝细胞内硷性及酸性磷酸酶含量并不随绝食时间延长而增多。3.重喂的肝细胞膨大,细胞质稀薄中空,血窦狭窄。这种改变在重喂11小时後即已出现,连喂3日并无恢复的迹象。中空的原因是由於糖元增多所致。

Adult albino mice were starved for 66 to 120 hours and the liver was examined forphosphatases,glycogen and ribonucleic acid.Alkaline phosphatase was found to increasemoderately while acid phosphatase showed no significant change.Both glycogen andribonucleic acid disappeared completely.The periphery of the hepatic lobule often stainedmore deeply than the centre due mainly to the conspicuous staining of the cell borderand of the Kupffer cells.The duration of starvation had no infiuence on the amountof both alkaline and acid phosphatases.In the refeeding experiment,mice starved for 48 to 72 hours were refed and werekilled 11 to 72 hours after subjecting to normal diet.The liver cells contained even greateramount of glycogen than in control animals.The restoration of ribonucleic acid was onlypartial.Both alkaline and acid phosphatases showed variable activities in the refeedingliver.The liver cells usually swelled and became rarefied,the sinusoids were very in-conspicuous.The swelling and rarefation of liver cells were caused by the accumulationof glycogen during refeeding.Three days refeeding did not bring this histological pictureto that of the control liver.