新颖栽培丹参材料的rDNA内外转录间隔区(ITS和ETS)分子标记鉴定

Molecular marker identification of rDNA internal and external transcribed spacer(ITS and ETS)of novel cultivated Danshen materials

本研究对前期发现的2个新颖多年生大紫花单株(V-HNYZ-bV-1和W-SXXA-bV-1)和1个二年生白花丹参(Salvia miltiorrhiza)栽培居群(W-SCHY-W-1)材料,进行了基于其rDNA内、外转录间隔区(ITS和ETS)的分子标记鉴定。结果表明:W-SXXA-bV-1和V-HNYZ-bV-1的ITS的GC含量均较高,W-SCHY-W-1的ITS和ETS的GC含量最低;新颖材料W-SXXA-bV-1和V-HNYZ-bV-1的ITS序列变异率较高,均高于W-SCHY-W-1,而对于ETS序列,W-SXXA-bV-1和V-HNYZ-bV-1接近(7.1%~6.5%),但低于W-SCHY-W-1(8.2%);W-SXXA-bV-1与V-HNYZ-bV-1亲缘关系较近,W-SXXA-bV-1、V-HNYZ-bV-1和W-SCHY-W-1均与Q-DZH-V-4亲缘关系较远,V-HNYZ-V-1与Q-DZH-V-4亲缘关系很近。上述结果与栽培形态观察结果都高度一致表明:W-SXXA-V-1和V-HNYZ-V-1是康定或甘西鼠尾草,新颖单株材料W-SXXA-bV-1可能为W-SXXA-V-1的突变体,V-HNYZ-V-1和Q-DZH-V-4同为荔枝草,V-HNYZ-bV-1可能为混杂在V-HNYZ-V-1中的康定或西藏鼠尾草,与W-SXXA-bV-1非常相似。W-SCHY-W-1为粘毛鼠尾草。

In this research,two novel perennial large purple-flowered monocots(V-HNYZ-bV-1 and W-SXXA-bV-1)and one biennial white-flowered Danshen(Salvia miltiorrhiza)in field cultivation(W-SCHY-W-1)were identified by molecular marker based on the internal and external transcribed spacers(ITS and ETS)of the rDNA.Results showed that the GC contents of ITS in W-SXXA-bV-1 and V-HNYZ-bV-1 were relatively high,while the GC contents of ITS and ETS in W-SCHY-W-1were the lowest.The ITS sequence variation rates of the novel materials W-SXXA-bV-1 and V-HNYZ-bV-1 was relatively high,and significantly higher than that of W-SCHY-W-1.The ETS sequence variation rates of W-SXXA-bV-1 and V-HNYZ-bV-1 were closer(7.1%-6.5%),but lower than that of the W-SCHY-W-1(8.2%).The relationship between W-SXXA-bV-1 and V-HNYZ-bV-1 was closer,the relationship between W-SXXA-bV-1/V-HNYZ-bV-1/W-SCHY-W-1 and Q-DZH-V-4 was relative distant,and the relationship between V-HNYZ-V-1 and Q-DZH-V-4 was the closest.The results were highly consistent with field cultivational observation.It is suggested that W-SXXA-V-1 and V-HNYZ-V-1 are S.prattii or S.przewalskii.The novel single plant W-SXXA-bV-1 is likely a mutant of W-SXXA-V-1.Both V-HNYZ-V-1 and Q-DZH-V-4 are S.plebeian.V-HNYZ-bV-1 is probably a material of S.prattii or S.wardii promiscuous in V-HNYZ-V-1,which is very similar to W-SXXA-bV-1.W-SCHY-W-1 is S.roborowskii.