miR-212-5p在肝癌组织中的表达及对HepG2细胞增殖、侵袭及迁移的影响

Expression of miR-212-5p in liver cancer tissues and its effect on HepG 2 cell

目的探讨miR-212-5p在肝癌组织中的表达及对HepG2细胞增殖、侵袭及迁移的影响。方法应用qRT-PCR法检测96例肝癌组织及癌旁组织中miR-212-5p表达水平,同时设HepG2细胞组、miR-212-5pmimics组与miR-212-5pinhibitor组。培养72h后,采用MTT法检测细胞活力;流式细胞仪检测细胞凋亡水平;Transwell实验和划痕实验检测细胞侵袭与迁移水平;qRT-PCR法检测细胞miR-212-5p表达水平。结果肝癌组织中miR-212-5p表达水平升高,肿瘤最大径≥2cm、TNM分期越高、浸润深度越深、病理级别越低、有淋巴结转移、有复发的肝癌患者miR-212-5p高表达率更高(P<0.05)。miR-212-5p模拟物能增强HepG2细胞增殖、侵袭及迁移能力,抑制HepG2细胞凋亡(P<0.05);miR-212-5p抑制剂能抑制HepG2细胞活力、增殖、侵袭及迁移能力,促进HepG2细胞凋亡(P<0.05)。结论miR-212-5p在肝癌组织中高表达,miR-212-5p可促进HepG2细胞增殖、侵袭及迁移能力,抑制HepG2细胞凋亡。

Objective To investigate the expression of miR-212-5p in hepatocellular carcinoma and its effect on the proliferation,invasion and migration of HepG2 cells.Methods The level of miR-212-5p in 96 cases of hepatocellular carcinoma tissues and adjacent tissues was detected by qRT-PCR.HepG2 cell group,miR-212-5p mimics group and miR-212-5p inhibitor group were set up.After 72h culture,the cell viability was detected by MTT assay.Apoptosis of cells was detected by flow cytometry.Invasion and migration of cells was detected by transwell test and scratch test.The level of miR-212-5p was detected by qRT-PCR.Results The level of miR-212-5p was increased in HCC tissues.The expression of miR-212-5p was higher in HCC patients with tumor maximum diameter≥2cm,higher TNM stage,deeper invasion depth,lower pathological grade,lymph node metastasis and recurrence(P<0.05).miR-212-5p mimics could enhance the proliferation,invasion and migration of HepG2 cells and inhibit the apoptosis of HepG2 cells(P<0.05).miR-212-5p inhibitor could inhibit the viability,proliferation,invasion and migration of HepG2 cells and promote the apoptosis of HepG2 cells(P<0.05).Conclusion miR-212-5p is highly expressed in HCC tissues,and miR-212-5p can promote the proliferation,invasion and migration of HepG2 cells,inhibit the apoptosis of HepG2 cells.