大豆GmEPSPS1基因表达及草甘膦抗性功能分析

Analysis of Expression of GmEPSPS1 Gene and Function of Glyphosate Resistance in Soybean

在田间种植大豆过程中,杂草对其产量影响很大,因此培育抗除草剂大豆新品种至关重要。在大豆中成功克隆了莽草酸途径关键酶基因GmEPSPS1,并分析了其进化关系及蛋白结构。荧光定量结果显示,在大豆不同组织中,GmEPSPS1基因在叶片中的相对表达量最高;并且大豆幼苗在草甘膦胁迫处理下,GmEPSPS1基因的表达呈现应激性升高,在植物体损伤后又表达下降。在大豆发状根中过表达GmEPSPS1基因发现,在草甘膦胁迫处理下,可导致发状根中莽草酸积累程度降低,且叶片中叶绿素含量提高,说明过表达GmEPSPS1基因可削弱草甘膦导致的杀伤作用,提高植物对草甘膦的耐受性。这为培育抗除草剂大豆新品系提供了候选基因。

Soybean is the important crop in China.Weeds have a great impact on its yield in the process of field planting.Therefore,it is very important to cultivate new herbicide-resistant soybean varieties.In this study,the key enzyme gene GmEPSPS1 of shikimate pathway was successfully cloned from soybean,and its evolution relationships and protein structure were then analyzed.The results of fluorescence quantitative PCR analysis showed that in different tissues of soybean,the relative expression level of the GmEPSPS1 gene in leaves was the highest.After soybean seedlings were treated with glyphosate,the relative expression level of the GmEPSPS1 gene increased first due to stress response,and then decreased after plant injury.Overexpression of the GmEPSPS1 gene in soybean hairy roots showed that under glyphosate stress,the accumulation of shikimic acid decreased in hairy roots and the content of chlorophyll increased in leaves.The results indicated that overexpression of GmEPSPS1 gene could attenuate glyphosate-induced cytotoxicity and improve plant tolerance to glyphosate.This study could provide candidated genes for the cultivation of new herbicide-resistant soybean varieties.