溶磷黑曲霉An510筛选鉴定及其溶磷机理

Screening and Identification of Phosphate-Soluble Aspergillus niger An510 and Its Mechanism of Phosphate Solubilization

为了活化土壤磷库资源,解决磷肥大量使用所产生的面源污染问题,以鉴别培养基从玉米根际土壤中分离到1株溶磷真菌An510,结合形态特征和ITS序列比对的方法对其进行分类鉴定,从不同碳源和氮源的角度探究其对溶磷量和pH的影响,采用高效液相色谱法分析了其发酵液中有机酸含量的变化。结果表明:溶磷真菌An510为黑曲霉(Aspergillus niger)。优先利用葡萄糖和硫酸铵进行溶磷,最高可达1366.50μg/mL,其发酵液中有机酸的种类和含量随培养时间的变化和营养基质的不同而不同;菌株An510可能通过产有机酸螯合发酵液中Ca2+,降低发酵液pH的方式溶磷。菌株An510具有较好的溶磷能力,在微生物肥料的开发方面具有潜在的应用价值。

To activate soil phosphorus pool resources and solve the problem of non-point source pollution caused by large-scale use of phosphate fertilizer,a phosphate-soluble fungus An510 was isolated from rhizosphere soil of maize by the medium,and identified by morphological characteristics and ITS sequence comparison.The effects of different carbon and nitrogen sources on phosphorus solubility and pH were investigated.The changes of organic acids in fermentation broth were analyzed by high performance liquid chromatography(HPLC).The results showed that the phosphate-soluble fungus An510 was Aspergillus niger.It could use glucose and ammonium sulfate preferentially to dissolve phosphorus,and the highest phosphorus solubility was 1366.50μg/mL.The type and content of organic acids in the fermentation broth varied with culture time and nutrient matrix.Strain An510 may dissolve phosphorus by producing organic acid to chelate Ca2+in the fermentation solution and reduce pH value of the fermentation solution.In conclusion,strain An510 has good phosphorus solubility and potential application value in the development of microbial fertilizers.