人参二醇组皂苷对对乙酰氨基酚致实验性肝损伤小鼠的保护作用

Protective Effect of the Pretreatment with Panaxadiol Saponins on Acetaminophen-Induced Experimental Liver Damaged Mice

为探讨人参二醇组皂苷(PDS)对对乙酰氨基酚致实验性肝损伤小鼠的保护作用及可能存在的机制。通过检测小鼠血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)的活性评价肝功,肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)的水平评价炎症,以及肝组织中还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)和丙二醛(MDA)水平的变化评价氧化应激,通过Hoechst 33258染色、凋亡抗体Bax免疫组化染色、Western blotting分析凋亡抗体Bax、Bcl-2、Cleaved-Caspase-3和氧化应激指标CYP2E1免疫荧光染色,检测PDS抑制肝细胞凋亡以及氧化应激的能力。结果表明:PDS能够通过抑制氧化应激、抗细胞凋亡、减少细胞炎症、减少组织坏死等方面抑制由对乙酰氨基酚所导致的肝损伤。

The activities of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were evaluated for liver function;the levels of tumor necrosis factor alpha(TNF-α)and interleukin 1 beta(IL-1β)were used to assess inflammation in the serum of mice;and the changes of the levels of reduced glutathione(GSH),superoxide dismutase(SOD)and malondialdehyde(MDA)in liver tissue were used to assess oxidative stress.Hoechst 33258 staining,apoptotic antibody Bax immunohistochemical staining and and Western blotting were used to analyze apoptotic antibodies Bax,Bcl-2 and Cleaved-Caspase-3 and oxidative stress index CYP2 E1 immunofluorescence staining,and the ability of PDS to inhibit hepatocyte apoptosis and oxidative stress was detected.The protective effects of panaxadiol saponins on acetaminophen-induced liver damaged mice and its possible mechanisms were investigated.The results showed that PDS can inhibit liver damage caused by acetaminophen by inhibiting oxidative stress,anti-apoptosis,reducing cell inflammation,and reducing tissue necrosis.