松辽黑猪OOEP基因多态性及其与繁殖性状的关联分析

Polymorphism of OOEP Gene and Its Association with Reproductive Traits in Songliao Black Pig

为探究OOEP基因多态性与松辽黑猪繁殖性状的关联性,选取130头松辽黑猪母猪为研究对象,利用Sanger直接测序法测序查找OOEP基因3个外显子的SNP位点,使用SPSS 19.0软件对OOEP基因SNP位点与松辽黑猪繁殖性状关联性进行分析。结果显示,松辽黑猪OOEP基因第2外显子100 bp和136 bp位点处存在SNPs位点,分别命名为C100T和A136C,在C100T位点上检测到3种基因型,分别为CC、CT和TT;在A136C位点上则检测到AA、AC和CC基因型。群体遗传参数分析及卡方适合性检验结果显示,在松辽黑猪群体中2个SNPs位点偏离Hardy-Weinberg平衡状态(P<0.01),C/T和A/C突变位点杂合度处于中等水平,属于中度多态(0.250.05),其中TT(CC)基因型在总产仔数、产活仔数及断奶数均高于CC(AA)和CT(AC)基因型。综上所述,OOEP基因第2外显子存在突变位点C/T和A/C,与松辽黑猪繁殖性状不存在显著差异,能否作为繁殖性状的遗传标记,需要在更大的群体进一步研究。

To explore the association of OOEP gene polymorphism with reproductive traits in Songliao Black Pig.130 sows were selected as the research object,and the SNP sites of three exons of OOEP gene were sequenced by Sanger direct sequencing method.The correlation analysis of OOEP gene SNP locus to reproductive traits of Songliao Black Pig was analyzed by SPSS 19.0 software.The results showed that SNPs were found in exon 2 of OOEP gene at 100 bp and 136 bp,named C100T and A136C,respectively.3 genotypes were detected at C100T locus,CC,CT and TT.AA,AC and CC genotypes were detected at A136C.The results of population genetic parameter analysis and chi square fitness test showed that the two SNPs sites deviated from hardy Weinberg equilibrium(P<0.01),and the heterozygosity of C/T and A/C mutation sites was at the middle level,which was moderate polymorphism(0.25<PIC<0.5),which could provide a little genetic information.The results of correlation analysis showed that the mutation sites of C/T and A/C in exon 2 of OOEP gene were found,The difference of the total number of piglets,the number of live piglets,the first birth weight,the weight of three weeks,the weaning weight,the number of weaning piglets and the number of nipples were not significant(P>0.05),TT(CC)genotype was higher than CC(AA)and CT(AC)genotype in total litter size,live litter size and weaning number.In conclusion,there are mutation sites C/T and A/C in exon 2 of OOEP gene,which is not significantly different from that of Songliao Black Pig.Whether it can be used as a genetic marker of reproductive traits needs further study in larger populations.