草原红牛Acot2基因的多态性分析

Polymorphism Analysis of Acot2 Gene in Grassland Red Cattle

为了研究草原红牛Acot2基因的多态性,为草原红牛育种工作中遗传标记的辅助选择提供参考数据,本试验随机选取127头草原红牛公牛为研究对象,提取血液DNA扩增Acot2基因全部外显子,以Sanger测序法获得扩增片段序列,通过序列比对,获得多态位点,分析草原红牛Acot2基因的多态性。结果表明:草原红牛Acot2基因chr10:84971902 bp处发生碱基A/G的突变,密码子由ACG变为GCG,在突变位点处,A是优势等位基因,AA是优势基因型,多态信息含量表明Acot2基因突变位点属于低度多态座位。碱基突变引起的错义突变导致原序列编码的苏氨酸变成了丙氨酸,推测可能引起蛋白质结构变化。

This experiment aimed to investigate the polymorphism of the Acot2 gene in the grassland red cattle,providing reference data for the auxiliary selection of genetic markers in the breeding of grassland red cattle.In this experiment,127 grassland red bulls were randomly selected as the research object,extracted blood DNA to amplify all exons of Acot2 gene,obtained the amplified fragment sequence by Sanger sequencing,obtained polymorphic sites through sequence alignment,and analyzed the polymorphism of Acot2 gene in grassland red cattle.The results showed that there was a mutation at the chr10:84971902 bp of Acot2 gene in grassland red cattle,the codon changed from ACG to GCG,at the mutation site,A was the dominant allele,AA was the dominant genotype,and the polymorphism information content showed that the mutation site of Acot2 gene belonged to low polymorphic locus.Missense mutations caused by base mutations lead to the conversion of threonine encoded by the original sequence to alanine,which may lead to structural changes of proteins.