分离、筛选和鉴定猪场污水污染物降解微生物及应用效果分析

Construction and Application of Compound Microbial Agent for Degrading Pollutants in Piggery Sewage

本研究利用分离、筛选获得降解猪场污水菌种,以分泌高酶活性的纤维素酶、蛋白酶、脂肪酶、淀粉酶、产酸微生物为指标,利用16S rDNA分析,通过NCBI系统比对,构建系统进化树,确定所属的种属鉴定菌为苏云金芽孢杆菌(Bacillus thuringiensis)。以养猪场污水为例,评估了分离、筛选获得的菌株处理对污水化学需氧量(COD)、生物需氧量(BOD5)、总磷(TP)、总氮(TN)、悬浮颗粒物(SS)去除污染物的效果,以及曝气增氧方式对污染物去除的影响。示范结果各项指标降解率分别为COD降解率为52.9%,BOD5降解率为60.5%,TP降解率为44%,TN降解率为35%,SS降解率为62%。证实添加筛选菌株、曝气增氧方式能有效降低水体污染物,为进一步开展应用奠定了基础。

In this study,bacteria strains of sewage in pig farm were isolated and screened from rotten manure,straw and other decomposed areas.Cellulase,protease,lipase,amylase and acid-producing microorganisms with high enzyme activity were used as indicators to identify bacteria as Bacillus thuringiensis by 16S rDNA analysis.Through NCBI system comparison,the phylogenetic tree was constructed to determine the species.Taking pig farm sewage as an example,the effects of isolated and screened strains on COD,BOD5,TP,TN and SS were evaluated.The demonstration results show that the aerated aeration method can effectively reduce water pollutants.The degradation rate of each index is COD 52.9%,BOD560.5%,TP 44%,TN 35%and SS 62%respectively.It was proved that adding screening strains and aeration with oxygen could effectively reduce water pollutants,which laid a foundation for further application.