大豆四粒荚突变体子房转录组分析

Transcriptome Analysis of Ovary in Soybean Four-pod Mutants

本研究首次以大豆四粒荚突变体为材料,采用Illumina/Solexa技术对未授粉子房进行转录组测序分析。结果显示:测序共获得55582个表达基因和2060个差异表达基因(differentially expressed genes,DEGs),其中上调DEGs 1381个,下调DEGs 679个,log2 ratio值大于10的DEGs 75个。GO和KEGG差异显著富集分析显示,差异基因几乎参与了如糖、脂肪、氨基酸、激素等所有主要物质的代谢、转录、翻译及信号转导等过程。该测序结果将为四粒荚相关基因的克隆及分子调控机理研究奠定基础,同时也对今后采用分子生物学手段培育优良大豆新品种具有重要理论意义。

In this study,four-pod mutants of soybean were used as materials for the first time to sequence and analyze the transcriptome of unpollinated ovary with Illumina/Solexa technology.The results showed that 55582 genes and 2060 differentially expressed genes(DEGs)were obtained by sequencing,of which 1381 were up regulated and 75 were DEGs with log2 ratio greater than 10.The analysis of significant enrichment of GO and KEGG showed that the differential genes were almost involved in the metabolism,transcription,translation and signal transduction of all major substances,such as sugar,fat,amino acids and hormones.The sequencing results will lay a foundation for the cloning of four-pod related genes and the study of molecular regulation mechanism,and also have important theoretical significance for the cultivation of new soybean varieties by molecular biological means in the future.