右归胶囊对肾阳虚大鼠固有免疫的影响及机制研究

Study on the Effect and Mechanism of Yougui Capsule on Innate Immunity of Rats with Kidney-yang Deficiency

目的本研究立足"肾为先天之本"的相关理论,通过肌注氢化可的松建立肾阳虚大鼠模型,以三基序蛋白(TRIM25)、视黄酸诱导基因-1(RIG-1)为指标探讨肾阳虚对机体固有免疫通路相关因子表达的影响。方法健康雄性SD大鼠60只,随机分为正常组、模型组、干预组,模型组和干预组肌肉注射氢化可的松建立肾阳虚大鼠模型,造模成功后,正常组和模型组给予生理盐水灌胃,干预组给予右归胶囊混悬液灌胃,干预结束后,检测尿17-羟皮质类固醇(17-OH-CS),检测血清睾酮(T)含量,取脾脏组织测定脾脏指数,Western Blot法检测TRIM25、RIG-1蛋白的表达,RT-PCR法检测TRIM25、RIG-1mRNA的表达。结果血液和尿液指标显示肾阳虚造模成功;第16天时,与正常组比较,模型组和干预组大鼠脾组织TRIM25、RIG-1表达增加,差异有统计学意义(P<0.05);第46天时,与模型组比较,干预组大鼠脾组织TRIM25、RIG-1表达较少,差异有统计学意义(P<0.05)。结论模型大鼠脾组织TRIM25、RIG-1的表达明显增加,表明肾阳虚大鼠体内免疫功能受到影响;温补肾阳名方-右归丸(右归胶囊)可以通过逆转模型大鼠脾组织TRIM25、RIG-1的表达,从而缓解肾阳虚对机体免疫功能失调的影响。

Objective Based on the relevant theory of"kidney as the innate basis",this study established a rat model of kidney-yang deficiency by muscle injection of hydrocortisone,and took TRIM25 and RIG-1 as indicators to explore the effect of kidney-yang deficiency on the expression of factors related to the innate immune pathway of the body.Methods 60 healthy male SD rats were randomly divided into normal group,model group,intervention group,model group and intervention group with muscle injection of hydrocortisone to establish the rat model of kidney-yang deficiency,after the successful modeling,normal group and model group were given saline lavage,intervention group was given Yougui capsule suspension liquid lavage.After the intervention,the contents of 17-OH-CS in urine,T contents in serum,spleen index in spleen tissue were detected,the expression of TRIM25,RIG-1 was tested by Western Blot,the expression of TRIM25,RIG-1 was tested by RT-PCR.Results The blood and urine indexes showed that the kidney-yang deficiency model was successfully constructed;on the 16 th day,TRIM25 and RIG-1 expression increased in spleen tissues of model group and intervention group compared with normal group,and the difference was statistically significant(P<0.05);on the 46 th day,TRIM25 and RIG-1 expression decreased in spleen tissues of intervention group compared with model group,and the difference was statistically significant(P<0.05).Conclusion The expression of TRIM25 and RIG-1 in spleen tissue of model rats significantly increased,indicating that the immune function of kidney-yang deficiency rats was affected.Yougui pill,a famous prescription for warming and tonifying kidney-yang,can reverse the expression of TRIM25 and RIG-1 in spleen tissue of model rats,so as to alleviate the effect of kidney-yang deficiency on immune dysfunction.