CHCHD7基因对急性髓系白血病患者预后的影响的生物信息分析

Bioinformatics analysis of effect of CHCHD7 gene on prognosis of patients with acute myeloid leukemia

目的探讨CHCHD7与急性髓系白血病(acute myeloid leukemia,AML)患者预后的相关性,从而揭示CHCHD7的表达水平与AML患者预后的关系,并预测出潜在的治疗AML的小分子药物。方法该研究从TARGET数据库获取下载130例AML患者的基因表达谱数据,通过生存分析、单因素及多因素COX回归分析探讨CHCHD7与患者预后的关系。随后利用功能富集分析来阐明CHCHD7在AML发病过程中潜在的生物学功能。最后通过Pearson相关分析和CMap分析找出潜在的治疗AML的药物。结果生存分析显示CHCHD7低表达组相对于CHCHD7高表达组的预后明显更好。单多因素回归分析证实CHCHD7可以作为AML患者预后不良的独立危险因素。富集分析结果显示CHCHD7位于膜筏、细胞膜微结构域等亚细胞定位并参与白细胞迁移等生物学功能。利用Pearson相关性分析并筛选与CHCHD7具有共表达关系的10个基因。最后,CMap分析结果显示酮舍林和苯氧苄胺可作为治疗AML的潜在药物。结论本研究首次揭示了CHCHD7的高表达为AML患者预后不良的高危因素,可以作为AML患者诊断和预后的潜在标志物。

Objective To investigate the correlation between CHCHD7 and the prognosis of patients with acute myeloid leukemia(AML),so as to reveal the relationship between the expression level of CHCHD7 and the clinical characteristics of AML patients,and to propose potential small molecule drugs for the treatment of AML.Methods The study obtained and downloaded the gene expression profile data of AML patients from target database,and analyzed the relationship between CHCHD7 and survival time by survival analysis,univariate and multivariate Cox regression analysis.CMap analysis was used to identify potential drugs for AML.Results Survival analysis showed that the prognosis of CHCHD7 low expression group was significantly better than that of CHCHD7 high expression group.Univariate and multivariate regression analysis confirmed that CHCHD7 could be an independent risk factor for poor prognosis in AML patients.The results of CMap showed that ketoserine and phenoxybenzylamine could be used as potential drugs in the treatment of AML.Conclusion The high expression of CHCHD7 is a high risk factor for poor prognosis of AML patients,which can be used as a potential marker for the diagnosis and prognosis of AML patients.